Purinergic Activation of Store-Operated Calcium Entry (SOCE) Regulates Cell Migration in Metastatic Ovarian Cancer Cells
Por:
Mata-Martinez, Esperanza, Gonzalez-Gallardo, Adriana, Diaz-Munoz, Mauricio, Vazquez-Cuevas, Francisco G. G.
Publicada:
1 jul 2023
Resumen:
Store-operated calcium entry (SOCE) is an important process in calcium
signaling. Its role in physiological and pathological events is well
recognized. However, in cancerous systems, the importance of SOCE in
relation to the degree of cancer aggressiveness, as well as its
regulation by ligands such as purinergic molecules, are not well
documented. This study aimed to characterize a differential effect of
the P2Y2 receptor (promoted by UTP of 10 & mu;M and inhibited by
ARC118925XX of 1 & mu;M) on intracellular calcium response between
metastatic (SKOV-3) and non-metastatic (CAOV-3) ovarian cell lines in
conditions of normal (1.5 mM) and zero extracellular calcium
concentration. The sustained calcium influx observed exclusively in
SKOV-3 cells was associated with the presence of SOCE (promoted by
thapsigargin (74.81 & PLUSMN; 0.94 & UDelta;F) and sensitive to 2-APB
(20.60 & PLUSMN; 0.85 & UDelta;F)), whereas its absence in CAOV-3
cells (26.2 & PLUSMN; 6.1 & UDelta;F) was correlated with a low
expression of ORAI1. The relevance of SOCE in metastatic SKOV-3 cells
was further corroborated when 2-APB significantly inhibited (40.4 &
PLUSMN; 2.8% of covered area) UTP-induced cell migration (54.6 &
PLUSMN; 3.7% of covered area). In conclusion, our data suggest that
SOCE activation elicited by the P2Y2 receptor is involved in the
aggressiveness of ovarian cancer cells.
Filiaciones:
Mata-Martinez, Esperanza:
Univ Nacl Autonoma Mexico, Dept Neurobiol Celular & Mol, Blvd Juriquilla 3001, Juriquilla 76230, Queretaro, Mexico
Gonzalez-Gallardo, Adriana:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Unidad Proteogen, Blvd Juriquilla 3001, Juriquilla 76230, Queretaro, Mexico
Diaz-Munoz, Mauricio:
Univ Nacl Autonoma Mexico, Dept Neurobiol Celular & Mol, Blvd Juriquilla 3001, Juriquilla 76230, Queretaro, Mexico
Vazquez-Cuevas, Francisco G. G.:
Univ Nacl Autonoma Mexico, Dept Neurobiol Celular & Mol, Blvd Juriquilla 3001, Juriquilla 76230, Queretaro, Mexico
gold, Gold
|