Bioactive bilayered dressing for compromised epidermal tissue regeneration with sequential activity of complementary agents
Por:
Reyes-Ortega F., Cifuentes A., Rodríguez G., Aguilar M.R., González-Gómez Á., Solis R., García-Honduvilla N., Buján J., García-Sanmartin J., Martínez A., Román J.S.
Publicada:
1 ene 2015
Resumen:
The article deals with the design, preparation, and evaluation of a new bilayered dressing for application in the healing of compromised wounds. The system is based on the sequential release of two complementary bioactive components to enhance the activation of the regeneration of dermal tissue. The internal layer is a highly hydrophilic and biodegradable film of gelatin and hyaluronic acid (HG), crosslinked with the natural compound genipin, which reacts with the amine groups of gelatin. This film is loaded with the proangiogenic, anti-inflammatory, and antibacterial peptide, proadrenomedullin N-terminal 20 peptide (PAMP), that is released slowly in the wound site. The external layer, more stable and less hydrophilic, is constituted by a biodegradable polyurethane derived from poly(caprolactone) and pluronic L61. This layer is loaded with resorbable nanoparticles of bemiparin (a fractionated low molecular weight heparin), which promotes the activation of growth factors, FGF and VEGF, and provides a good biomechanical stability and controlled permeability of the bilayered dressing. Experiments carried out in mice demonstrate the excellent angiogenic effect of the HG film in the dermal tissue. Application of the bilayered dressing in the wound healing rabbit ear model shows an improved cicatrization of the wound in both ischemic and non-ischemic defects, favoring epithelialization and reducing noticeably the contraction and the inflammation. © 2015 Acta Materialia Inc.
Filiaciones:
Reyes-Ortega F.:
Biomaterials Group, Institute of Polymer Science and Technology (ICTP-CSIC), Madrid, Spain
Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
Cifuentes A.:
Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
Department of Medical Specialities, School of Medicine, University of Alcalá, Alcalá de Henares, Madrid, Spain
Rodríguez G.:
Biomaterials Group, Institute of Polymer Science and Technology (ICTP-CSIC), Madrid, Spain
Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
Aguilar M.R.:
Biomaterials Group, Institute of Polymer Science and Technology (ICTP-CSIC), Madrid, Spain
Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
González-Gómez Á.:
Biomaterials Group, Institute of Polymer Science and Technology (ICTP-CSIC), Madrid, Spain
Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
Solis R.:
Biomaterials Group, Institute of Polymer Science and Technology (ICTP-CSIC), Madrid, Spain
García-Honduvilla N.:
Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
Department of Medical Specialities, School of Medicine, University of Alcalá, Alcalá de Henares, Madrid, Spain
Buján J.:
Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
Department of Medical Specialities, School of Medicine, University of Alcalá, Alcalá de Henares, Madrid, Spain
García-Sanmartin J.:
Angiogenesis Study Group, Oncology Area, Center for Biomedical Research of la Rioja (CIBIR), Logroño, Spain
Martínez A.:
Angiogenesis Study Group, Oncology Area, Center for Biomedical Research of la Rioja (CIBIR), Logroño, Spain
Román J.S.:
Biomaterials Group, Institute of Polymer Science and Technology (ICTP-CSIC), Madrid, Spain
Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain
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