Inhibition of proliferation, migration, and adhesion of skin fibroblasts by enzymatic poly(gallic acid) grafted with L-Arginine
Por:
Ortega-Sanchez, Carmina, Perez-Diaz, Mario Alberto, Martinez-Lopez, Valentin, Zacaula-Juarez, Noe, Gonzalez-Torres, Maykel, Leyva-Gomez, Gerardo, Hernandez-Valdepena, Miguel Angel, Gimeno, Miquel, Sanchez-Sanchez, Roberto
Publicada:
1 ene 2023
Resumen:
Psoriasis and atopic dermatitis (AD) are characterized by enhanced skin
inflammation, which results in hyperproliferation and the recruitment of
immune cells into the skin. For that reason, it is needed a chemical
capable to reduce cell proliferation and the recruitment of cells. The
search for new molecules for therapeutic skin treatment mainly focuses
on the antioxidant and anti-inflammatory properties, highlighting the
rheological properties of polymeric polypeptides. We studied L-arginine
(L-Arg) grafted (-g-) to enzymatic poly(gallic acid) (PGAL). The latter
is a multiradical antioxidant with greater properties and thermal
stability. The derivative was enzymatically polymerized in an innocuous
procedure. The poly(gallic acid)-g-L-Arg molecule (PGAL-g-L-Arg)
inhibits bacterial strains which also have been involved in the
progression of psoriasis and AD. However, it is important to analyze
their biological effect on skin cells. The cell viability was analyzed
by calcein/ethidium homodimer assays and crystal violet. The
proliferation and cell attachment were determined by a curve of time and
quantitation of the optical density of crystal violet. To analyze the
cell migration a wound-healing assay was performed. This synthesis
demonstrates that it is not cytotoxic at high concentrations (250 mu
g/mL). We observed a decrease in the proliferation, migration, and
adhesion of dermal fibroblasts in vitro but the compound could not avoid
the increase of reactive oxygen species in the cell. Based on our
findings, PGAL-g-L-Arg is a promising candidate for treating skin
diseases such as psoriasis and AD where decreasing the proliferation and
cell migration could help to avoid inflammation.
Filiaciones:
Ortega-Sanchez, Carmina:
Laboratorio de Biotecnología, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Ciudad de México, 14389, Mexico
Perez-Diaz, Mario Alberto:
Laboratorio de Biotecnología, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Ciudad de México, 14389, Mexico
Martinez-Lopez, Valentin:
Unidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Ciudad de México, 14389, Mexico
Zacaula-Juarez, Noe:
Laboratorio de Biotecnología, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Ciudad de México, 14389, Mexico
Gonzalez-Torres, Maykel:
Laboratorio de Biotecnología, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Ciudad de México, 14389, Mexico
Leyva-Gomez, Gerardo:
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria ,Circuito Exterior S/N ,Del. Coyoacán, Ciudad de México, 04510, Mexico
Hernandez-Valdepena, Miguel Angel:
Facultad de Química, Departamento de Alimentos y Biotecnologí a, Universidad Nacional Autó noma de Mé xico, Ciudad Universitaria, Ciudad de México, 04510, Mexico
Gimeno, Miquel:
Facultad de Química, Departamento de Alimentos y Biotecnologí a, Universidad Nacional Autó noma de Mé xico, Ciudad Universitaria, Ciudad de México, 04510, Mexico
Sanchez-Sanchez, Roberto:
Unidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Ciudad de México, 14389, Mexico
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