Root microbiome diversity and structure of the Sonoran desert buffelgrass (Pennisetum ciliare L.)
Por:
Jara-Servin A., Silva A., Barajas H., Cruz-Ortega R., Tinoco-Ojanguren C., Alcaraz L.D.
Publicada:
1 ene 2023
Categoría:
Multidisciplinary
Resumen:
Buffelgrass (Pennisetum ciliare) is an invasive plant introduced into Mexico's Sonoran desert for cattle grazing and has converted large areas of native thorn scrub. One of the invasion mechanisms buffelgrass uses to invade is allelopathy, which consists of the production and secretion of allelochemicals that exert adverse effects on other plants' growth. The plant microbiome also plays a vital role in establishing invasive plants and host growth and development. However, little is known about the buffelgrass root-associated bacteria and the effects of allelochemicals on the microbiome. We used 16S rRNA gene amplicon sequencing to obtain the microbiome of buffelgrass and compare it between samples treated with root exacknudates and aqueous leachates as allelochemical exposure and samples without allelopathic exposure in two different periods. The Shannon diversity values were between H' = 5.1811-5.5709, with 2,164 reported bacterial Amplicon Sequence Variants (ASVs). A total of 24 phyla were found in the buffelgrass microbiome, predominantly Actinobacteria, Proteobacteria, and Acidobacteria. At the genus level, 30 different genera comprised the buffelgrass core microbiome. Our results show that buffelgrass recruits microorganisms capable of thriving under allelochemical conditions and may be able to metabolize them (e.g., Planctomicrobium, Aurantimonas, and Tellurimicrobium). We also found that the community composition of the microbiome changes depending on the developmental state of buffelgrass (p = 0.0366; ANOSIM). These findings provide new insights into the role of the microbiome in the establishment of invasive plant species and offer potential targets for developing strategies to control buffelgrass invasion. Copyright: © 2023 Jara-Servin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Filiaciones:
Jara-Servin A.:
Laboratorio de Genómica Ambiental, Departamento de Biología Celular, Universidad Nacional Autónoma de México, Facultad de CienciasMexico City, Mexico
Posgrado en Ciencias Bioquímicas, Universidad Nacional Autónoma de MéxicoMexico City, Mexico
Silva A.:
Departamento de Ecología de la Biodiversidad, Instituto de Ecología, Universidad Nacional Autónoma de México, Hermosillo, Sonora, Mexico
Barajas H.:
Laboratorio de Genómica Ambiental, Departamento de Biología Celular, Universidad Nacional Autónoma de México, Facultad de CienciasMexico City, Mexico
Cruz-Ortega R.:
Departamento de Ecología Funcional, Instituto de Ecología, Universidad Nacional Autónoma de MéxicoMexico City, Mexico
Tinoco-Ojanguren C.:
Departamento de Ecología de la Biodiversidad, Instituto de Ecología, Universidad Nacional Autónoma de México, Hermosillo, Sonora, Mexico
Alcaraz L.D.:
Laboratorio de Genómica Ambiental, Departamento de Biología Celular, Universidad Nacional Autónoma de México, Facultad de CienciasMexico City, Mexico
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