Genetic polymorphism at an odorant receptor gene (Or39) among mosquitoes of the Anopheles gambiae complex in Senegal (West Africa)
Por:
Arnal A., Kengne P., Brengues C., Dabire K.R., Diabate A., Bassene H., Simard F.
Publicada:
1 ene 2014
Resumen:
Background: Olfaction plays a significant role in insect behavior during critical steps of their life-cycle, such as host-seeking during foraging or the search for a mate. Here, we explored genetic polymorphism within and divergence between sibling species of the African malaria mosquito, Anopheles gambiae sensu lato in the gene sequence and encoded peptides of an odorant receptor, Or39. This study included sympatric specimens of An. gambiae sensu stricto, An. coluzzii and An. arabiensis sampled together in the village of Dielmo, Senegal. Results: A 1,601 bp genomic sequence composed of 6 exons and 5 introns was obtained for Or39 from 6-8 mosquitoes in each of the 3 species. DNA sequence analysis revealed a high level of molecular polymorphism (p = 0.0154; Haplotype diversity = 0.867) and high overall genetic differentiation between taxa (Fst > 0.92, P < 0.01). In total, 50 parsimony informative sites were recorded. Throughout the whole dataset, there were 13 non-synonymous mutations resulting in aminoacid changes in the encoded protein. Each of the 6 different identified peptides was species-specific and none was shared across species. Most aminoacid changes were located on the intracellular domains of the protein. However, intraspecific polymorphisms in An. gambiae and An. arabiensis as well as species-specific mutations also occurred in the first extracellular domain. Conclusions: Although obtained from a limited number of specimens, our results point towards genetic differences between cryptic species within the An. gambiae complex in a gene of biological relevance that might be of evolutionary significance when exposed to disruptive selective forces. © 2014 Arnal et al.; licensee BioMed Central Ltd.
Filiaciones:
Arnal A.:
Institut de Recherche Pour le Développement (IRD), UMR IRD, Team BEES (Biology, Ecology and Evolution of Vector Systems), 911 Avenue Agropolis, Montpellier, Cedex 5 34394, France
Kengne P.:
Institut de Recherche Pour le Développement (IRD), UMR IRD, Team BEES (Biology, Ecology and Evolution of Vector Systems), 911 Avenue Agropolis, Montpellier, Cedex 5 34394, France
Organisation de Coordination Pour la Lutte Contre les Endémies en Afrique Centrale (OCEAC), P.O. Box 288, Yaoundé, Cameroon
Brengues C.:
Institut de Recherche Pour le Développement (IRD), UMR IRD, Team BEES (Biology, Ecology and Evolution of Vector Systems), 911 Avenue Agropolis, Montpellier, Cedex 5 34394, France
Dabire K.R.:
Institut de Recherche en Sciences de la Santé (IRSS), P.O. Box 545, Bobo-Dioulasso, Burkina Faso
Diabate A.:
Institut de Recherche en Sciences de la Santé (IRSS), P.O. Box 545, Bobo-Dioulasso, Burkina Faso
Bassene H.:
UMR 198 URMITE, Campus International IRD/UCAD de Hann, Dakar, Senegal
Simard F.:
Institut de Recherche Pour le Développement (IRD), UMR IRD, Team BEES (Biology, Ecology and Evolution of Vector Systems), 911 Avenue Agropolis, Montpellier, Cedex 5 34394, France
Gold
|