Lysophosphatidic acid receptor LPA1 trafficking and interaction with Rab proteins, as evidenced by Förster resonance energy transfer
Por:
Martinez-Morales, Juan Carlos, Gonzalez-Ruiz, Karla D., Romero-Avila, M. Teresa, Rincon-Heredia, Ruth, Reyes-Cruz, Guadalupe, Garcia-Sainz, Jesus Adolfo
Publicada:
1 ene 2023
Ahead of Print:
1 abr 2023
Resumen:
LPA1 internalization to endosomes was studied employing Förster Resonance Energy Transfer (FRET) in cells coexpressing the mCherry-lysophosphatidic acid LPA1 receptors and distinct eGFP-tagged Rab proteins. Lysophosphatidic acid (LPA)-induced internalization was rapid and decreased afterward: phorbol myristate acetate (PMA) action was slower and sustained. LPA stimulated LPA1-Rab5 interaction rapidly but transiently, whereas PMA action was rapid but sustained. Expression of a Rab5 dominant-negative mutant blocked LPA1-Rab5 interaction and receptor internalization. LPA-induced LPA1-Rab9 interaction was only observed at 60 min, and LPA1-Rab7 interaction after 5 min with LPA and after 60 min with PMA. LPA triggered immediate but transient rapid recycling (i.e., LPA1-Rab4 interaction), whereas PMA action was slower but sustained. Agonist-induced slow recycling (LPA1-Rab11 interaction) increased at 15 min and remained at this level, whereas PMA action showed early and late peaks. Our results indicate that LPA1 receptor internalization varies with the stimuli. © 2023 Elsevier B.V.
Filiaciones:
Martinez-Morales, Juan Carlos:
Departamento de Biología Celular y Desarrollo, Ap. Postal 70-600, Ciudad de México, 04510, Mexico
Dept Biol Celular & Desarrollo, Ap Postal 70-600, Ciudad De Mexico 04510, Mexico
Gonzalez-Ruiz, Karla D.:
Departamento de Biología Celular y Desarrollo, Ap. Postal 70-600, Ciudad de México, 04510, Mexico
Dept Biol Celular & Desarrollo, Ap Postal 70-600, Ciudad De Mexico 04510, Mexico
Romero-Avila, M. Teresa:
Departamento de Biología Celular y Desarrollo, Ap. Postal 70-600, Ciudad de México, 04510, Mexico
Dept Biol Celular & Desarrollo, Ap Postal 70-600, Ciudad De Mexico 04510, Mexico
Rincon-Heredia, Ruth:
Unidad de Imagenología, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ap. Postal 70-600, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Unidad Imagenol, Inst Fisiol Celular, Ciudad Univ,Ap Postal 70-600, Ciudad De Mexico 04510, Mexico
Reyes-Cruz, Guadalupe:
Departamento de Biología Celular, Centro de Investigación y Estudios Avanzados-Instituto Politécnico Nacional, Colonia San Pedro Zacatenco, Ciudad de México, 07360, Mexico
Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Biol Celular, Ciudad De Mexico 07360, Mexico
Garcia-Sainz, Jesus Adolfo:
Departamento de Biología Celular y Desarrollo, Ap. Postal 70-600, Ciudad de México, 04510, Mexico
Dept Biol Celular & Desarrollo, Ap Postal 70-600, Ciudad De Mexico 04510, Mexico
UNAM, Inst Fisiol Celular, Ciudad Univ,Ap Postal 70-248, Ciudad De Mexico 04510, Mexico
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