Curcumin-loaded microemulsion: formulation, characterization, and in vitro skin penetration
Por:
Luna-Canales, Irene Carolina, Delgado-Buenrostro, Norma Laura, Chirino Y.I., Nava-Arzaluz, Guadalupe, Piñón-Segundo E., Martinez-Cruz, Graciela, Ganem-Rondero, Adriana
Publicada:
1 mar 2023
Ahead of Print:
1 mar 2023
Resumen:
Objective: Formulation of curcumin in a microemulsion with a high
loading capacity and that favors its penetration into the
skin.SignificanceTake advantage of the properties of microemulsions to
promote the penetration of curcumin into the skin, with the aim of
enhancing its therapeutic effects.
Methods: Curcumin was formulated in microemulsions based on oleic acid
(oil phase), Tween((R)) 80 (surfactant), and Transcutol((R)) HP
(cosurfactant). The microemulsion formation area was mapped by
constructing pseudo-ternary diagrams for surfactant:co-surfactant ratios
1:1, 1:2, and 2:1. Microemulsions were characterized through
measurements of specific weight, refractive index, conductivity,
viscosity, droplet size, and in vitro skin permeation studies.
Results: Nine microemulsions were prepared and characterized, showing
clear, stable formulations with globule size dependent on the proportion
of the components. The microemulsion with the highest loading capacity
(60 mg/mL), based on Tween((R)) 80, Transcutol((R)) HP, oleic acid, and
water (40:40:10:10) was able to penetrate the viable epidermis, finding
a total amount of curcumin in the receptor medium at 24 h of 10.17 +/-
9.7 mu g/cm(2). The distribution of curcumin in the skin, visualized by
confocal laser scanning microscopy, showed that the maximum amount was
located between 20 and 30 mu m.
Conclusion: The inclusion of curcumin in a microemulsion allows its
passage into and through the skin. The localization of curcumin,
especially in the viable epidermis, would be important for those cases
where local conditions are sought to be treated.
Filiaciones:
Luna-Canales, Irene Carolina:
Laboratorio de Investigación y Posgrado en Tecnología Farmacéutica (L-322, Campo 1), Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Mexico
Univ Nacl Autonoma Mexico, Lab Invest, Campo 1, Cuautitlan, Mexico
Univ Nacl Autonoma Mexico, Posgrado Tecnol Farmaceut L 322, Campo 1, Cuautitlan, Mexico
Delgado-Buenrostro, Norma Laura:
Laboratorio 10, Unidad de Biomedicina, Universidad Nacional Autónoma de México, Tlalnepantla, Mexico
Univ Nacl Autonoma Mexico, Unidad Biomed, Lab 10, Tlalnepantla, Mexico
Chirino Y.I.:
Laboratorio 10, Unidad de Biomedicina, Universidad Nacional Autónoma de México, Tlalnepantla, Mexico
Nava-Arzaluz, Guadalupe:
Laboratorio de Investigación y Posgrado en Tecnología Farmacéutica (L-322, Campo 1), Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Mexico
Univ Nacl Autonoma Mexico, Lab Invest, Campo 1, Cuautitlan, Mexico
Univ Nacl Autonoma Mexico, Posgrado Tecnol Farmaceut L 322, Campo 1, Cuautitlan, Mexico
Piñón-Segundo E.:
Laboratorio de Sistemas Farmacéuticos de Liberación Modificada (L-13, UIM), Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Mexico
Martinez-Cruz, Graciela:
Laboratorio de Reometría, Nave 3000, Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Mexico
Univ Nacl Autonoma Mexico, Lab Reometria, Nave 3000, Cuautitlan, Mexico
Ganem-Rondero, Adriana:
Laboratorio de Investigación y Posgrado en Tecnología Farmacéutica (L-322, Campo 1), Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Mexico
Univ Nacl Autonoma Mexico, Lab Invest, Campo 1, Cuautitlan, Mexico
Univ Nacl Autonoma Mexico, Posgrado Tecnol Farmaceut L 322, Campo 1, Cuautitlan, Mexico
Univ Nacl Autonoma Mexico, Lab Sistemas Farmaceut Liberac Modificada L 13, UIM, Cuautitlan, Mexico
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