Alterations on the morphology, nitric oxide synthesis and activity of platelets reproduced in rats as possible biomarkers for depression are reversed by fluoxetine
Por:
González-Trujano M.E., Alvarado-Vásquez N., Mendoza-Sotelo J., López G., Estrada-Camarena E., Martínez-Mota L., Moreno J.
Publicada:
1 ene 2012
Resumen:
Biochemical markers associated with the prognosis of depression in humans are being described in the literature, whereas experimental studies in animal models in search for antidepressant strategies are lacking. The aim of this study was to evaluate platelet morphology, platelet activity and nitric oxide (NO) synthesis as possible biomarkers of depressive-like behavior by using FST alone and in the presence of fluoxetine. Naïve rats were compared to those receiving vehicle or fluoxetine at 10 mg/kg i.p. in acute, subchronic and chronic administration in the FST. After behavioral assessment, platelets were isolated from blood samples and analyzed by flow cytometry to determine the platelet mitochondrial membrane potential and NO synthesis. In addition, HPLC and electron microscopy were used to examine 5-HT and tryptophan levels and morphology of platelets, respectively. Rats receiving vehicle and exposed to FST showed depressive-like behavior at all the times tested; after chronic FST rats showed a similar pattern of alteration in platelet morphology and in the studied as possible biochemical markers as those previously recognized in depressive humans. Depressive-like behavior in rats exposed to FST was prevented in the presence of fluoxetine administration at all the times tested and associated with the prevention of alterations in platelet morphology, platelet activity and NO synthesis, and/or in 5-HT concentrations. The results of the present study suggest that platelet function and morphology might be relevant markers for the prognosis of depression and the search for functional treatments. Besides, the relevance of FST as model to study this psychiatric illness is reinforced. © 2012 Elsevier Inc. All rights reserved.
Filiaciones:
González-Trujano M.E.:
Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calz, Mexico-Xochimilco 101, Col. Sn Lorenzo Huipulco, 14370, México, D.F., Mexico
Alvarado-Vásquez N.:
Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Calz, de Tlalpan 4502, Col. Sección XVI, 14080, México, D.F., Mexico
Mendoza-Sotelo J.:
Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calz, Mexico-Xochimilco 101, Col. Sn Lorenzo Huipulco, 14370, México, D.F., Mexico
López G.:
Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calz, Mexico-Xochimilco 101, Col. Sn Lorenzo Huipulco, 14370, México, D.F., Mexico
Estrada-Camarena E.:
Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calz, Mexico-Xochimilco 101, Col. Sn Lorenzo Huipulco, 14370, México, D.F., Mexico
Martínez-Mota L.:
Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calz, Mexico-Xochimilco 101, Col. Sn Lorenzo Huipulco, 14370, México, D.F., Mexico
Moreno J.:
Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calz, Mexico-Xochimilco 101, Col. Sn Lorenzo Huipulco, 14370, México, D.F., Mexico
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