Chronic Consumption of Moringa Leaf Powder (Moringa oleifera) Concentration-Dependent Effects in a Drosophila melanogaster Type 2 Diabetes Model


Por: Lopez-Rodriguez, Norma A., Sanchez-Ortiz, Laura K., Reynoso-Camacho, Rosalia, Riesgo-Escovar, Juan R., Loarca-Pina, Guadalupe

Publicada: 3 abr 2023
Resumen:
Objective The metabolic effects of chronic consumption of food laced with different doses of moringa leaf powder (MLP) were assessed using a heteroallelic mutant of the sole insulin receptor gene of Drosophila melanogaster (InR), and the yellow,white (y,w) control stock. Methods The MLP composition was partially determined. Both strains were raised in a standard diet (SD) or in a SD supplemented with different MLP doses (0.5, 1.5, 2.5, 4.0, and 5.5%) until 4-5 days of emergence. Afterward, the total carbohydrate, lipid, glucose, and triacylglyceride levels were measured in the flies. Additionally, survival and weight changes were reported. For metabolic tests, female and male virgin flies were evaluated separately. Results Low MLP supplementation improved carbohydrate and glucose levels in the y,w strain. Additionally, the InR-mutant strain reported lower lipid content when subjected to the same regimes. Survival improved in both strains with low MLP doses, while chronic consumption of high MLP doses resulted in triacylglycerides increase, weight gain, and survival reduction. Conclusion Low doses of MLP supplementation improves some metabolic parameters that affect flies' survival, especially in the y,w strain. Furthermore, the same low doses of MLP treatments also resulted in metabolic improvements in the InR-mutant flies; however, MLP consumption levels should be carefully assessed. Supplemental data for this article is available online at

Filiaciones:
Lopez-Rodriguez, Norma A.:
 Programa de Posgrado en Alimentos del Centro de la República, Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, Centro Universitario, Qro, Santiago de Querétaro, Mexico

 Univ Autonoma Queretaro, Ctr Univ, Sch Chem, Santiago De Queretaro, Qro, Mexico

Sanchez-Ortiz, Laura K.:
 Programa de Posgrado en Alimentos del Centro de la República, Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, Centro Universitario, Qro, Santiago de Querétaro, Mexico

 Univ Autonoma Queretaro, Ctr Univ, Sch Chem, Santiago De Queretaro, Qro, Mexico

Reynoso-Camacho, Rosalia:
 Programa de Posgrado en Alimentos del Centro de la República, Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, Centro Universitario, Qro, Santiago de Querétaro, Mexico

 Univ Autonoma Queretaro, Ctr Univ, Sch Chem, Santiago De Queretaro, Qro, Mexico

Riesgo-Escovar, Juan R.:
 Departamento de Neurobiología del Desarrollo y Neurofisiología, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro, Juriquilla, Mexico

 Univ Nacl Autonoma Mex, Dept Neurobiol Desarrollo & Neurofisiol, Inst Neurobiol, Juriquilla, Queretaro, Mexico

 Univ Nacl autonoma mex, Dept Neurobiol Desarrollo & neurofisiol, Inst Neurobiol, Juriquilla 76230, Queretaro, Mexico

Loarca-Pina, Guadalupe:
 Programa de Posgrado en Alimentos del Centro de la República, Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, Centro Universitario, Qro, Santiago de Querétaro, Mexico

 Univ Autonoma Queretaro, Ctr Univ, Sch Chem, Santiago De Queretaro, Qro, Mexico

 Univ autonoma Queretaro, Ctr Univ, Sch Chem, cerro campanas S-N, Santiago De Queretaro 76010, Qro, Mexico
ISSN: 27697061





Journal Of The American Nutrition Association
Editorial
Routledge, 2-4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND
Tipo de documento: Article
Volumen: 42 Número: 3
Páginas: 285-294
WOS Id: 000933746600006
ID de PubMed: 35512766