Light and focused ion beam microscopy workflow for resin-embedded tissues
Por:
Merchant-Larios, Horacio, Giraldo-Gomez, David M., Castro-Dominguez, Adriana, Marmolejo-Valencia, Alejandro
Publicada:
25 ene 2023
Resumen:
Although the automated image acquisition with the focused ion beam
scanning electron microscope (FIB-SEM) provides volume reconstructions,
volume analysis of large samples remains challenging. Here, we present a
workflow that combines a modified sample protocol of the classical
transmission electron microscope with FIB-SEM volume imaging. The
proposed workflow enables efficient 3D structural surveys of rabbit
ovaries collected at consecutive developmental stages. The precise
trimming of the region of interest adds the time dimension to the
volume, constructing a virtual 4D electron microscopy. We found
filopodia-like processes emitted by oocyte cysts allowing contact
between oocytes not previously observed.
Filiaciones:
Merchant-Larios, Horacio:
Instituto de Investigaciones Biomédicas Departamento de Biología Celular y Fisiología, Universidad Nacional Autónoma de México (UNAM), México City, Mexico
Giraldo-Gomez, David M.:
Carl Zeiss de México S. A. de C. V, Research Microscopy Solutions, México City, Mexico
Castro-Dominguez, Adriana:
Instituto de Investigaciones Biomédicas Departamento de Biología Celular y Fisiología, Universidad Nacional Autónoma de México (UNAM), México City, Mexico
Marmolejo-Valencia, Alejandro:
Instituto de Investigaciones Biomédicas Departamento de Biología Celular y Fisiología, Universidad Nacional Autónoma de México (UNAM), México City, Mexico
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