Insulin-like growth factor 1 ( IGF-1) concentrations in synovial fluid of sound and osteoarthritic horses, and its correlation with proinflammatory cytokines IL-6 and TNF alpha
Por:
Fernando García-Lacy F., Méndez-Cruz S.T., Reyes-Vivas, Horacio, Dávila-Borja V.M., Barrera-Morales J.A., Gutierrez-Ospina, Gabriel, Gomez-Chavarin, Margarita, Trigo-Tavera F.J.
Publicada:
1 ene 2023
Resumen:
Insulin-like growth factor I (IGF-1) is the most important known growth
factor for cartilage repair in horses. It promotes mitosis of
chondrocytes, collagen II expression, and extra cellular matrix
production. Osteoarthritis (OA) is the most common musculoskeletal
condition that causes lameness and poor performance in sport horses. A
total of 11 lame horses were clinically and radiographically evaluated,
and all were confirmed to suffer a front metacarpophalangeal lameness by
a positive flexion test, a low-4-point nerve block and an intraarticular
block. Total protein, IGF-1, IL-6 and TNF. were determined by ELISA,
demonstrating changes and different correlations between clinical
condition, radiographic changes and degree of inflammation. All horses
with joint associated pain and therefore associated lameness,
demonstrated a significant increase of total protein (P<0.0001) and
IGF-1 concentration (P<0.05). Concentrations of IL- 6 and TNF. between
controls and lame horses demonstrated significant differences (P<0.01
and P<0.001 respectively). Horses with less radiographic changes,
demonstrated the highest IGF-1 expression in synovial fluid, and horses
with more chronic OA conditions had very similar IGF-1 expression levels
than control joints. In all lame joints, it was identified by Western
blot a lighter isoform of IGF-1 (similar to 7.5 kDa) which was
inflammation related and it is the molecular weight of the mature
peptide, and all control joints expressed a heavier isoform (similar to
12 kDa). This finding could lead to new research for sequencing and
targeting the isoform which is not expressed during an inflammatory
process within a joint, and to have a better understanding of its role
in the horse's joint.
Filiaciones:
Fernando García-Lacy F.:
Universidad Nacional Autónoma de México, Facultad de Medicina Veterinaria y Zootecnia, Departamento de Medicina, Cirugía y Zootecnia para Équidos, Ciudad de México, Mexico
Méndez-Cruz S.T.:
Instituto Nacional de Pediatría, Laboratorio de Bioquímica Genética, Ciudad de México, Mexico
Reyes-Vivas, Horacio:
Instituto Nacional de Pediatría, Laboratorio de Bioquímica Genética, Ciudad de México, Mexico
Dávila-Borja V.M.:
Instituto Nacional de Pediatría, Laboratorio de Oncología Experimental, Mexico
Barrera-Morales J.A.:
SEDENA, Centro Ecuestre de Alto Rendimiento, Ciudad México, Mexico
Gutierrez-Ospina, Gabriel:
Universidad Nacional Autónoma de México, Departamento de Fisiología, Instituto de Investigaciones Biomédicas, Ciudad de México, Mexico
Gomez-Chavarin, Margarita:
Universidad Nacional Autónoma de México, Facultad de Medicina, Departamento de Fisiología, Ciudad de México, Mexico
Trigo-Tavera F.J.:
Universidad Nacional Autónoma de México, Facultad de Medicina Veterinaria y Zootecnia, Departamento de Patología, Ciudad de México, Mexico
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