Molecular detection of a fragment of the bluetongue virus in sheep from different regions of Mexico
Por:
Anaya E.R., Ceron-Tellez, Fernando, Yáñez-Garza L.A., Gutiérrez-Hernández J.L., Sarmiento-Salas R.E., Loza-Rubio, Elizabeth
Publicada:
1 ene 2023
Resumen:
Bluetongue disease (BTD) affects various species of wild and domestic
ruminants. In Mexico, the disease, caused by the bluetongue virus (BTV)
is still regarded as exotic, despite the fact that antibodies have been
detected on several occasions. The objective was to establish molecular
techniques using a synthetic gene, including the genes NS1 and NS3 as
positive controls for the diagnosis of BTV in samples of sheep from
different regions of the country. A total of 320 total whole blood
samples were obtained from sheep. The samples obtained were evaluated by
end-point RT-PCR and real-time RT-PCR, the conditions having been
established by the work group. Twelve sheep samples were found to be
positive for the detection of NS1; these samples were sequenced,and a
fragmentof 101 base pairs was obtained. Upon alignment, wereobtained
identities with sequences reported in GenBank with NS1 fragments ranging
from 89 % (p= 1e-12) to 98 % (p= 4e-13), corresponding to serotypes
10, 11 and 12.From these samples, two positive sheep samples were
obtained usingreal-time PCR (RT-PCR):one from Chiapas (Chiapas
breed),and the other,from Tamaulipas (Suffolk breed). The results of the
RT-PCR were corroborated by CPA-SENASICA. This work provides evidence,
for the first time in Mexico, of the importance of using a synthetic
gene as a positive control to perform BSL-2 detection in official
laboratories, which in a health emergency is of utmost importance.
Filiaciones:
Anaya E.R.:
Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP), Centro Nacional de Recursos Genéticos, Mexico
Ceron-Tellez, Fernando:
INIFAP, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), Campus Ciudad de México. Carretera México-Toluca Km 15.5, Colonia Palo Alto., Alcaldía Cuajimalpa de Morelos, Ciudad de México, 05110, Mexico
Yáñez-Garza L.A.:
Universidad Nacional Autónoma de México, FMVZ, Mexico
Gutiérrez-Hernández J.L.:
INIFAP, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), Campus Ciudad de México. Carretera México-Toluca Km 15.5, Colonia Palo Alto., Alcaldía Cuajimalpa de Morelos, Ciudad de México, 05110, Mexico
Sarmiento-Salas R.E.:
Universidad Nacional Autónoma de México, FMVZ, Mexico
Loza-Rubio, Elizabeth:
INIFAP, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), Campus Ciudad de México. Carretera México-Toluca Km 15.5, Colonia Palo Alto., Alcaldía Cuajimalpa de Morelos, Ciudad de México, 05110, Mexico
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