Selective Regional Isolation of Brain Microvessels
Por:
Medina-Flores F., Hurtado-Alvarado G., Gómez-González B.
Publicada:
1 ene 2021
Resumen:
The study of the regionalized function of the blood-brain barrier at the level of brain endothelial cells and pericytes is essential to understand the biological properties and molecular mechanisms regulating this biological barrier. The isolation of blood vessels from specific brain regions will allow to understand regional differences in susceptibility to pathological phenomena such as ischemia, traumatic brain injury, and neurodegenerative diseases, such as Alzheimer disease. Here, we propose an efficient and fast method to isolate brain endothelial cells and pericytes from a specific cerebral region. The isolated brain endothelial cells and pericytes are viable to perform conventional molecular and histological techniques such as Western blots, immunocytofluorescence, and scanning electron microscopy. © 2020, Springer Science+Business Media New York.
Filiaciones:
Medina-Flores F.:
Area of Neurosciences, Department Biology of Reproduction, CBS, Mexico City, Mexico
Posgrado en Biología Experimental, Universidad Autónoma Metropolitana, Unidad Iztapalapa, Mexico City, Mexico
Hurtado-Alvarado G.:
Area of Neurosciences, Department Biology of Reproduction, CBS, Mexico City, Mexico
Gómez-González B.:
Area of Neurosciences, Department Biology of Reproduction, CBS, Mexico City, Mexico
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