Ultrastructural changes in giant cell tumor of bone cultured cells exposed to quercetin
Por:
Estrada-Villaseñor E., Delgado-Cedillo A., Hernández-Pérez A., Meneses A., Olivos Meza A., Hidalgo-Bravo A., Santamaría M., Landa Solís C.
Publicada:
1 ene 2021
Resumen:
Giant cell tumor of bone (GCTB) is a primary bone tumor that affects skeletally mature people and whose main treatment is surgical. Because there are few pharmacological alternatives for the treatment of this tumor to find other molecules or compounds that could be potential therapeutic agents is desirable. Quercetin is a flavonoid with described antitumoral effect in different types of cancer cell lines that could be a possible option in GCTB treatment. However, there is no literature about the effect of quercetin on GCTB. In the present paper, we reported the ultrastructural changes in GCTB cells exposed to quercetin and also determined the expression of RIP1K, Caspase 3 and Caspase 8 on the exposed cells. For this purpose, GCTB sample was obtained from one patient and cultured. Quercetin affected all the histological components of the GCTB. The ultrastructural changes consisted mainly in necroptosis, autophagocytosis and secondary necrosis. This is the first report about quercetin effects on giant cell tumor of bone cultured cells. Further studies in other models could be done to support the use of quercetin as a complementary treatment in giant cell tumor of bone. Abbreviations: Giant cell tumor of bone (GCTB); transmission electron microscopy (TEM); reverse transcription–polymerase chain reaction (RT-PCR); receptor interacting protein kinase 1 (RIP1K); Dulbecco’s Modified Eagle’s Medium (DMEM). © 2021 Taylor & Francis Group, LLC.
Filiaciones:
Estrada-Villaseñor E.:
Pathology Service, National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra, México City, Mexico
Delgado-Cedillo A.:
Bone Tumors Service, National Institute of Rehabilitation, Mexico City, Mexico
Hernández-Pérez A.:
Electronic Microscopy Laboratory, National Institute of Rehabilitation, Mexico City, Mexico
Meneses A.:
Pathology Service, National Cancer Institute, Mexico City, Mexico
Olivos Meza A.:
Arthroscopy Service, National Institute of Rehabilitation, Mexico City, Mexico
Hidalgo-Bravo A.:
Genetic Laboratory, National Institute of Rehabilitation Luis Guillermo Ibarra, México City, Mexico
Santamaría M.:
Genetic Laboratory, National Institute of Rehabilitation Luis Guillermo Ibarra, México City, Mexico
Landa Solís C.:
Tissue Engineering and Cell Therapy Unit, National Institute of Rehabilitation, Mexico City, Mexico
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