Curcumin-loaded Polycaprolactone/Collagen Composite Fibers as Potential Antibacterial Wound Dressing
Por:
San Martin-Martinez, E., Casanas-Pimentel, R., Almaguer-Flores, A., Prado-Prone, G., Garcia-Garcia, A., Landa-Solis, C., Hernandez-Rangel, A.
Publicada:
1 nov 2022
Resumen:
The development of wound dressings with therapeutical benefits is of
great importance in skin tissue engineering applications, adding
bioactive molecules into biomaterials is a strategy to achieve a better
biological response. In this study, four different concentrations of
curcumin (CUR; 5, 10, 15 and 20 by weight in relation to the PCL
content) were incorporated into solutions composed of polycaprolactone
(PCL) and collagen (COL) for the manufacture of electrospun fibers. The
PCL-COL-CUR fibers were physicochemically characterized in terms of
their morphology, wettability, degradation rate, mechanical behavior,
and cumulative curcumin release. The in vitro biological properties of
the composite membranes were also evaluated. The results indicated that
the membranes have diameters on average of approximately 200 nm. The
water uptake was adequate for exudates remotion in a wound, and the
degradation rate of the fibers was highly appropriate to achieve
complete skin tissue regeneration. The addition of CUR to composite
membranes produced a significant increase in the mechanical properties
which indicate a satisfactory clinical handling. The incorporation of
CUR produced a significant decrease in the planktonic growth of S.
aureus over time, however, the antibacterial effect against E. coli was
limited, the presence of CUR did not cause the inhibition of its growth.
Finally, the viability of human dermal fibroblasts seeded on the top of
the membranes indicated the cytotoxic dosage effect of CUR, the two
highest CUR concentrations produced a significant loss of cell
viability. Overall, our results suggested that the CUR-loaded PCL-COL
composite membranes are promising candidates for use as antibacterial
dressings to enhance clinical wound management.
Filiaciones:
San Martin-Martinez, E.:
Natl Polytech Inst, Ctr Res Appl Sci & Adv Technol, CICATA Legaria, Legaria 694, Mexico City 11500, DF, Mexico
Casanas-Pimentel, R.:
Natl Polytech Inst, Ctr Res Appl Sci & Adv Technol, CICATA Legaria, Legaria 694, Mexico City 11500, DF, Mexico
Almaguer-Flores, A.:
Univ Nacl Autonoma Mexico, Fac Dent, Postdoctoral & Res Div, Circuito Exterior S-N,Ciudad Univ, Mexico City 04510, DF, Mexico
Prado-Prone, G.:
Univ Nacl Autonoma Mexico, Fac Dent, Postdoctoral & Res Div, Circuito Exterior S-N,Ciudad Univ, Mexico City 04510, DF, Mexico
Garcia-Garcia, A.:
Ctr Res Adv Mat CIMAV Monterrey, Grp Synth & Modificat Nanostruct & Bidimens Mat, PIIT, Apodaca 66628, NL, Mexico
Landa-Solis, C.:
Natl Inst Rehabil Luis Guillermo Ibarra Ibarra, Tissue Engn Unit, Calz Mexico Xochimilco 289, Mexico City 14389, DF, Mexico
Hernandez-Rangel, A.:
(Corresponding Author), Natl Polytech Inst, Ctr Res Appl Sci & Adv Technol, CICATA Legaria, Legaria 694, Mexico City 11500, DF, Mexico
Natl Polytech Inst, Ctr Res Appl Sci & Adv Technol, CICATA Legaria, Legaria 694, Mexico City 11500, DF, Mexico
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