GC/MS Analysis, Antioxidant Activity, and Antimicrobial Effect of Pelargonium peltatum (Geraniaceae)
Por:
Alonso A.-M., Reyes-Maldonado O.K., Puebla-Pérez A.M., Arreola M.P.G., Velasco-Ramírez S.F., Zúñiga-Mayo V., Sánchez-Fernández R.E., Delgado-Saucedo J.-I., Velázquez-Juárez G.
Publicada:
1 ene 2022
Resumen:
In recent years, the increase in antibiotic resistance demands searching for new compounds with antimicrobial activity. Phytochemicals found in plants offer an alternative to this prob-lem. The genus Pelargonium contains several species; some have commercial use in traditional med-icine such as P. sinoides, and others such as P. peltatum are little studied but have promising potential for various applications such as phytopharmaceuticals. In this work, we characterized the freeze-dried extracts (FDEs) of five tissues (root, stem, leaf, and two types of flowers) and the ethyl acetate fractions from leaf (Lf-EtOAc) and flower (Fwr-EtOAc) of P. peltatum through the analysis by thin-layer chromatography (T.L.C.), gas chromatography coupled to mass spectrometry (GC-MS), phy-tochemicals quantification, antioxidant capacity, and antimicrobial activity. After the first round of analysis, it was observed that the FDE-Leaf and FDE-Flower showed higher antioxidant and anti-microbial activities compared to the other FDEs, for which FDE-Leaf and FDE-Flower were frac-tionated and analyzed in a second round. The antioxidant activity determined by ABTS showed that Lf-EtOAc and Fwr-EtOAc had the lowest IC50 values with 27.15 ± 1.04 and 28.11 ± 1.3 µg/mL, respectively. The content of total polyphenols was 264.57 ± 7.73 for Lf-EtOAc and 105.39 ± 4.04 mg G.A./g FDE for Fwr-EtOAc. Regarding the content of flavonoid, Lf-EtOAc and Fw-EtOAc had the highest concentration with 34.4 ± 1.06 and 29.45 ± 1.09 mg Q.E./g FDE. In addition, the minimum inhibitory concentration (M.I.C.) of antimicrobial activity was evaluated: Lf-EtOAc and Fwr-EtOAc were effective at 31.2 µg/mL for Staphylococcus aureus and 62.5 µg/mL for Salmonella enterica, while for the Enterococcus feacalis strain, Fwr-EtOAc presented 31.2 µg/mL of M.I.C. According to the GC-MS analysis, the main compounds were 1,2,3-Benzenetriol (Pyrogallol), with 77.38% of relative abundance in the Lf-EtOAc and 71.24% in the Fwr-EtOAc, followed by ethyl gallate (13.10%) in the Fwr-EtOAc and (Z)-9-Octadecenamide (13.63% and 6.75%) in both Lf-EtOAc and Fwr-EtOAc, re-spectively. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Filiaciones:
Alonso A.-M.:
Doctorado en Ciencias en Procesos Biotecnológicos, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Jalisco, Guadalajara, CP 44340, Mexico
Reyes-Maldonado O.K.:
Centro Universitario de Ciencias Exactas e Ingenierías, Laboratorio de Bioquímica Avanzada, Departamento de Química, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Jalisco, Guadalajara, CP 44430, Mexico
Puebla-Pérez A.M.:
Centro Universitario de Ciencias Exactas e Ingenierías, Departamento de Farmacobiología, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Jalisco, Guadalajara, CP 44430, Mexico
Arreola M.P.G.:
Centro de Investigación Biomédica de Occidente, División de Genética, I.M.S.S., Sierra Mojada 800, Independencia Oriente, Jalisco, Guadalajara, CP 44340, Mexico
Velasco-Ramírez S.F.:
Centro Universitario de Ciencias Exactas e Ingenierías, Laboratorio de Bioquímica Avanzada, Departamento de Química, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Jalisco, Guadalajara, CP 44430, Mexico
Zúñiga-Mayo V.:
Campus Montecillo, CONACyT-Instituto de Fitosanidad, Colegio de Postgraduados, Estado de Mexico, Texcoco, 56230, Mexico
Sánchez-Fernández R.E.:
Laboratorio Nacional de Investigación y Servicio Agroalimentario y Forestal (LANISAF), Universidad Autónoma Chapingo, Mexico-Texcoco km 38.5, Chapingo, Texcoco de Mora, 56230, Mexico
Delgado-Saucedo J.-I.:
Centro Universitario de Ciencias Exactas e Ingenierías, Departamento de Farmacobiología, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Jalisco, Guadalajara, CP 44430, Mexico
Velázquez-Juárez G.:
Centro Universitario de Ciencias Exactas e Ingenierías, Laboratorio de Bioquímica Avanzada, Departamento de Química, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Jalisco, Guadalajara, CP 44430, Mexico
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