Detection of Biomarkers Associated with Acute Kidney Injury by a Gold Nanoparticle Based Colloidal Nano-Immunosensor by Fourier-Transform Infrared Spectroscopy with Principal Component Analysis
Por:
Ortega-Hernández N., Ortega-Romero M., Medeiros-Domingo M., Barbier O.C., Rojas-López M.
Publicada:
1 ene 2022
Resumen:
A colloidal nano-immunosensor based on gold nanoparticles covered in their surface with protein A and the immunoglobulin anti-kidney injury molecule-1 was prepared and tested in human urine to identify a biomarker that diagnose acute kidney injury. The urine was obtained from patients with cancer treated with cisplatin, diagnosed with acute kidney injury, and from healthy individuals. Fourier-transform infrared spectroscopy analysis showed important spectral differences between both types of groups, primarily for the intervals from 1750 to 1500 cm-1 and from 1000 to 891 cm-1 where the amide I group (C = O) and the glycosylated functional groups of this protein absorb. The principal component analysis applied to the vibrational signals obtained with the nano-immunosensor allows adequate discrimination of urine from patients with acute kidney injury from samples from healthy individuals. The simplicity of the proposed method, from the preparation of the nano-immunosensor to the spectral and chemometric analysis, may make this method an interesting alternative to practically diagnose acute kidney injury using a small volume of urine. © 2022 Taylor & Francis Group, LLC.
Filiaciones:
Ortega-Hernández N.:
Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Ex Hacienda de San Juan Molino, Tlaxcala, Tepetitla, 90700, Mexico
Ortega-Romero M.:
Departamento de Toxicología, Cinvestav, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, Mexico
Unidad de Investigación y Diagnóstico en Nefrología y Metabolismo Mineral Óseo, Hospital Infantil de México Federico Gómez (HIMFG), Ciudad de México, Mexico
Medeiros-Domingo M.:
Unidad de Investigación y Diagnóstico en Nefrología y Metabolismo Mineral Óseo, Hospital Infantil de México Federico Gómez (HIMFG), Ciudad de México, Mexico
Barbier O.C.:
Departamento de Toxicología, Cinvestav, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, Mexico
Rojas-López M.:
Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada, Ex Hacienda de San Juan Molino, Tlaxcala, Tepetitla, 90700, Mexico
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