Presencia de la enzima ß-glucuronidasa de neutrófilos en pacientes con esporotricosis
Por:
Palma-Ramos A., Tepexpan-Chavarría K., González-Hernández M.C., Monroy-Núñez A., Castrillón-Rivera L.E., Castañeda-Sánchez J.I., Vega-Memije E., Arenas-Guzmán R.
Publicada:
1 ene 2021
Categoría:
Dermatology
Resumen:
BACKGROUND: Sporotrichosis is a chronic fungal infection caused by the Sporothrix schenckii complex, characterized by a suppurative, tuberculoid or foreign body granulomas, formed by histiocytes that surround a central area of neutrophils that contribute to the nonspecific immunity system, in these cells it is found the enzyme ß-glucuronidase that catalyzes the breakdown of complex carbohydrates, hydrolyzing ß-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides. OBJECTIVE: To determine the presence of the enzyme ß-glucuronidase from neutrophils in histological sections of biopsies of seven patients diagnosed with sporotrichosis by the Dermatology and Mycology Department at Manuel Gea González General Hospital of public health. MATERIAL AND METHOD: A prospective study done from January to December 2019 in which seven patient histopathological samples were used and three cuts per sample were made for the performance of the techniques: HE, PAS and for the labeling of ß-glucuronidase; the commercial package Cell and Tissue Staining Kit, Goat Kit HRP-AEC System was used (catalog number CTS009) R&D Systems, and the primary antibody was goat human anti-ß-glucuronidase polyclonal IgG by the Santa Cruz Biotechnology laboratories. RESULTS: It was found the presence of ß-glucuronidase adhered to the yeasts of Sporothrix sp, previously observed by the PAS reaction in all the samples. CONCLUSIONS: We found ß-glucuronidase from neutrophil polymorphonuclear, adhered to the cell wall of the yeasts present in the histological sections of the seven patients with sporotrichosis. © 2021 Comunicaciones Cientificas Mexicanas S.A. de C.V.. All rights reserved.
Filiaciones:
Palma-Ramos A.:
Laboratorio de Inmunobiología, Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Ciudad de México, Mexico
Tepexpan-Chavarría K.:
Laboratorio de Inmunobiología, Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Ciudad de México, Mexico
González-Hernández M.C.:
Laboratorio de Inmunobiología, Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Ciudad de México, Mexico
Monroy-Núñez A.:
Laboratorio de Inmunobiología, Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Ciudad de México, Mexico
Castrillón-Rivera L.E.:
Laboratorio de Inmunobiología, Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Ciudad de México, Mexico
Castañeda-Sánchez J.I.:
Laboratorio de Inmunobiología, Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Ciudad de México, Mexico
Vega-Memije E.:
Departamento de Dermatologia, Hospital General Dr. Manuel Gea Gonzalez, Ciudad de Mexico, Mexico
Arenas-Guzmán R.:
Sección de Micología, Hospital General Dr. Manuel Gea González, Ciudad de México, Mexico
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