Effect of Shock Waves on the Growth of Aspergillus niger Conidia: Evaluation of Germination and Preliminary Study on Gene Expression
Por:
Larrañaga-Ordaz D., Martinez-Maldonado, Miguel A., Millan-Chiu, Blanca E., Fernandez, Francisco, Castaño-Tostado E., Gomez-Lim, Miguel angel, Loske, Achim M.
Publicada:
1 nov 2022
Resumen:
Shock waves, as used in medicine, can induce cell permeabilization,
genetically transforming filamentous fungi; however, little is known on
the interaction of shock waves with the cell wall. Because of this, the
selection of parameters has been empirical. We studied the influence of
shock waves on the germination of Aspergillus niger, to understand their
effect on the modulation of four genes related to the growth of conidia.
Parameters were varied in the range reported in protocols for genetic
transformation. Vials containing conidia in suspension were exposed to
either 50, 100 or 200 single-pulse or tandem shock waves, with different
peak pressures (approximately 42, 66 and 83 MPa). In the tandem mode,
three delays were tested. To equalize the total energy, the number of
tandem ``events'' was halved compared to the number of single-pulse
shock waves. Our results demonstrate that shock waves do not generate
severe cellular effects on the viability and germination of A. niger
conidia. Nevertheless, increase in the aggressiveness of the treatment
induced a modification in four tested genes. Scanning electron
microscopy revealed significant changes to the cell wall of the conidia.
Under optimized conditions, shock waves could be used for several
biotechnological applications, surpassing conventional techniques.
Filiaciones:
Larrañaga-Ordaz D.:
Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico
Martinez-Maldonado, Miguel A.:
Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico
Millan-Chiu, Blanca E.:
Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico
CONACyT—Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico
Fernandez, Francisco:
Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico
Castaño-Tostado E.:
Facultad de Química, Universidad Autónoma de Querétaro, C.U., Cerro de las Campanas s/n, Querétaro, 76010, Mexico
Gomez-Lim, Miguel angel:
Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, Irapuato, 36824, Mexico
Loske, Achim M.:
Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro, 76230, Mexico
Green Published, gold, Green Submitted, Gold, Green
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