Antimicrobial and anti-inflammatory activity of Cystatin C on human gingival fibroblast incubated with Porphyromonas gingivalis
Por:
Blancas-Luciano B.E., Becker-Fauser, Ingeborg, Zamora-Chimal, Jaime, Delgado-Dominguez, Jose, Ruiz-Remigio, Adriana, Leyva-Huerta E.R., Portilla-Robertson, Javier, Fernández-Presas A.M.
Publicada:
25 oct 2022
Resumen:
Background. Periodontal disease is considered one of the most prevalent
chronic infectious diseases, often leading to the disruption of
tooth-supporting tissues, including alveolar bone, causing tooth
mobility and loss. Porphyromonas gingivalis is considered the major
etiological agent of this disease, having a plethora of virulence
factors, including, lipopolysaccharides (LPS), hemolysins, and
proteinases. Antimicrobial peptides are one of the main components of
the innate immune response that inhibit the growth of P. gingivalis. The
aim of this study was to analyze the antimicrobial activity of
cystatinCand to assess the effect on the inflammatory and
anti-inflammatory cytokines, the production of reactive oxygen species,
and in the release of nitric oxide by human gingival fibroblasts
incubated with P. gingivalis in the presence and absence of cystatin C.
Methods. P. gingivalis ATCC 33277 was exposed to cystatin C for 24h and
co-cultured with human gingival fibroblasts (HGFs) ATCC CRL-2014. The
effect of cystatin on growth of P. gingivalis and HGFs was evaluated.
Pro-inflammatory (TNF ff, IL-1 fi) and anti-inflammatory (IL-10)
cytokines were determined by ELISA in the supernatants of HGFs incubated
with P. gingivalis exposed to cystatin C. Additionally, nitrites and
reactive oxygen species (ROS) production were evaluated. Results.
Cystatin Cinhibited the growth of P. gingivalis without affecting HGFs.
Incubation of HGFs with P. gingivalis led to a significant increase of
TNF- alpha and IL-1 beta. In contrast, HGFs incubated with P. gingivalis
exposed to cystatin C showed a decreased production of both cytokines,
whereas IL-10 was enhanced. Incubation of HGFs with P. gingivalis led to
an increase of nitric oxide (NO) and ROS production, which was reduced
in the presence of the peptide. Conclusions. Cystatin C inhibits the
growth of P. gingivalis and decreases the inflammatory cytokines, ROS,
and NO production during infection of HGFs with P. gingivalis. Knowledge
on the antimicrobial and immunomodulatory properties of cystatin C could
aid in the design of new therapeutic approaches to facilitate the
elimination of this bacterium to improve the treatment of periodontal
disease.
Filiaciones:
Blancas-Luciano B.E.:
Departamento de Microbiología y Parasitología, Universidad Nacional Autónoma de México, México City, Mexico
Becker-Fauser, Ingeborg:
Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México, Mexico City, Mexico
Zamora-Chimal, Jaime:
Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México, Mexico City, Mexico
Delgado-Dominguez, Jose:
Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México, Mexico City, Mexico
Ruiz-Remigio, Adriana:
Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México, Mexico City, Mexico
Leyva-Huerta E.R.:
Departmento de Medicina Oral y Patología, División de Posgrado, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City, Mexico
Portilla-Robertson, Javier:
Departmento de Medicina Oral y Patología, División de Posgrado, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico City, Mexico
Fernández-Presas A.M.:
Departamento de Microbiología y Parasitología, Universidad Nacional Autónoma de México, México City, Mexico
Centro de investigación en Ciencias de la Salud (CICSA), Universidad Anáhuac México Campus Norte, Mexico City, Mexico
Green Published, gold, Gold, Green
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