Recent developments in generation of marker-free transgenic plants
Por:
Singh R.K., Sharma L., Bohra N., Anandhan S., Ruiz-May E., Quiroz-Figueroa F.R.
Publicada:
1 ene 2019
Resumen:
A plant modified through artificial insertion of a foreign DNA into its genome is referred to as “genetically modified plant” or a “transgenic” plant. The selection of the transgenic tissues during the genetic transformation process is based on the constitutively expressed marker gene(s) coding for reporters, such as those conferring resistance against antibiotics and/or herbicides. In this direction, Agrobacterium-mediated genetic co-transformation is arguably the most commonly used technique to transfer the gene(s) of interest as well as the marker gene(s). However, the latter is purposeless once a transgenic tissue has been selected. Although these marker genes are important for screening purposes, they exhibit safety concerns for the environment as well as among consumers. At times, commercial transgenic plants transfer these gene(s) to the weeds or other organisms, leading to the development of resistance among nontarget plants. Moreover, the escape of such gene could affect the wild relatives or land races via gene flow. Therefore, in order to maintain sustainability, removing the marker gene(s) from a transgenic crop is of utmost importance, prior to its commercialization. Hitherto, several methodologies have been evolved for the development of a marker-free transgenic crop. In the present summary, we discuss the merits and the shortcomings of the Agrobacterium-mediated genetic co-transformation. In addition, we review the recent developments among other approaches and their impacts and suggest directions for their maximum utilization in the near future. © Springer Nature Singapore Pte Ltd. 2019.
Filiaciones:
Singh R.K.:
Centro de Química de Vila Real (CQ-VR), Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal
Sharma L.:
CITAB - Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, UTAD, Vila Real, Portugal
Bohra N.:
School of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, UTAD, Vila Real, Portugal
Department of Biotechnology, National Institute of Technology, Warangal, Telangana, India
Anandhan S.:
ICAR- Directorate of Onion and Garlic Research, Rajgurunagar, Pune, Maharashtra, India
Ruiz-May E.:
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C, ClusterBioMimic®, Xalapa, Veracruz, Mexico
Quiroz-Figueroa F.R.:
Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Sinaloa (CIIDIR-IPN Unidad Sinaloa), Laboratorio de Fitomejoramiento Molecular, Guasave, Sinaloa, Mexico
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