Oxidative Stress, Cytotoxic and Inflammatory Effects of Urban Ultrafine Road-Deposited Dust from the UK and Mexico in Human Epithelial Lung (Calu-3) Cells
Por:
Hammond, Jessica, Maher, Barbara A., Gonet, Tomasz, Bautista, Francisco, Allsop, David
Publicada:
1 sep 2022
Resumen:
Road-deposited dust (RD) is a pervasive form of particulate pollution
identified (typically via epidemiological or mathematical modelling) as
hazardous to human health. Finer RD particle sizes, the most abundant
(by number, not mass), may pose greater risk as they can access all
major organs. Here, the first in vitro exposure of human lung epithelial
(Calu-3) cells to 0-300 mu g/mL of the ultrafine (<220 nm) fraction of
road dust (UF-RDPs) from three contrasting cities (Lancaster and
Birmingham, UK, and Mexico City, Mexico) resulted in differential
oxidative, cytotoxic, and inflammatory responses. Except for Cd, Na, and
Pb, analysed metals were most abundant in Mexico City UF-RDPs, which
were most cytotoxic. Birmingham UF-RDPs provoked greatest ROS release
(only at 300 mu g/mL) and greatest increase in pro-inflammatory cytokine
release. Lancaster UF-RDPs increased cell viability. All three UF-RDP
samples stimulated ROS production and pro-inflammatory cytokine release.
Mass-based PM limits seem inappropriate given the location-specific PM
compositions and health impacts evidenced here. A combination of new,
biologically relevant metrics and localised regulations appears critical
to mitigating the global pandemic of health impacts of particulate air
pollution and road-deposited dust.
Filiaciones:
Hammond, Jessica:
Univ Lancaster, Fac Hlth & Med, Div Biomed & Life Sci, Lancaster LA1 4YQ, England
Maher, Barbara A.:
Univ Lancaster, Ctr Environm Magnetism & Palaeomagnetism, Lancaster Environm Ctr, Lancaster LA1 4YQ, England
Gonet, Tomasz:
Univ Lancaster, Ctr Environm Magnetism & Palaeomagnetism, Lancaster Environm Ctr, Lancaster LA1 4YQ, England
Jaguar Land Rover, Lighthorne Heath CV35 0BJ, Warwick, England
Bautista, Francisco:
Univ Nacl Autonoma Mexico, Ctr Invest Geog Ambiental, Lab Univ Geofis Ambiental, Morelia 58190, Michoacan, Mexico
Allsop, David:
Univ Lancaster, Fac Hlth & Med, Div Biomed & Life Sci, Lancaster LA1 4YQ, England
gold, Green Published
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