De Novo Polycomb Recruitment and Repressive Domain Formation
Por:
Alejandra Hernandez-Romero, Itzel, Julian Valdes, Victor
Publicada:
1 sep 2022
Resumen:
Every cell of an organism shares the same genome; even so, each cellular
lineage owns a different transcriptome and proteome. The Polycomb group
proteins (PcG) are essential regulators of gene repression patterning
during development and homeostasis. However, it is unknown how the
repressive complexes, PRC1 and PRC2, identify their targets and elicit
new Polycomb domains during cell differentiation. Classical recruitment
models consider the pre-existence of repressive histone marks; still, de
novo target binding overcomes the absence of both H3K27me3 and
H2AK119ub. The CpG islands (CGIs), non-core proteins, and RNA molecules
are involved in Polycomb recruitment. Nonetheless, it is unclear how de
novo targets are identified depending on the physiological context and
developmental stage and which are the leading players stabilizing
Polycomb complexes at domain nucleation sites. Here, we examine the
features of de novo sites and the accessory elements bridging its
recruitment and discuss the first steps of Polycomb domain formation and
transcriptional regulation, comprehended by the experimental
reconstruction of the repressive domains through time-resolved genomic
analyses in mammals.
Filiaciones:
Alejandra Hernandez-Romero, Itzel:
Natl Autonomous Univ Mexico UNAM, Inst Cellular Physiol IFC, Dept Cell Biol & Dev, Mexico City 04510, DF, Mexico
Julian Valdes, Victor:
Natl Autonomous Univ Mexico UNAM, Inst Cellular Physiol IFC, Dept Cell Biol & Dev, Mexico City 04510, DF, Mexico
hybrid, Green Published
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