G protein-coupled estrogen receptor (GPER/GPR30) levels in pelvic floor muscles and its association with estrogen status in female rabbits
Por:
Rodriguez-Jaimes, Sharet Y., Hernandez-Hernandez, Guadalupe C., Hernandez-Aragon, Laura G., Sanchez-Garcia, Octavio, Martinez-Gomez, Margarita, Cuevas-Romero, Estela, Castelan, Francisco
Publicada:
2 sep 2022
Resumen:
Objective: To assess the relative expression of the G-protein coupled
estrogen receptor (GPER) in the bulbospongiosus (Bsm) and pubococcygeus
(Pcm) muscles in control, ovariectomized (OVX), and OVX with estradiol
benzoate supplementation (OVX + EB) rabbits.Methods: We used tissues
from C, 1-month OVX, and OVX plus 15-day EB implanted (OVX + EB) groups.
The GPER expression was evaluated by Western blot and
immunohistochemistry for both Bsm and Pcm. Results: Both muscles showed
a GPER immunoreactivity in blood vessels, inside myofibers next to
myonuclei, and in polymorphonuclear cells. Four-week ovariectomy did not
modify the GPER expression in the Bsm and Pcm, but two-week estradiol
benzoate increased it in the latter muscle alone.Conclusions: We
demonstrated that the Bsm and Pcm of female rabbits express GPER. High
serum estradiol levels elevate GPER relative expression in the Pcm
alone. The present study supports the remarkable estrogen sensitivity of
the Pcm.
Filiaciones:
Rodriguez-Jaimes, Sharet Y.:
Univ Autonoma Tlaxcala, Ctr Tlaxcala Biol Conducta, Tlaxcala, Mexico
Univ Autonoma Tlaxcala, Ciencias Biol, Tlaxcala, Mexico
Hernandez-Hernandez, Guadalupe C.:
Univ Autonoma Tlaxcala, Ctr Tlaxcala Biol Conducta, Tlaxcala, Mexico
Univ Autonoma Tlaxcala, Ciencias Biol, Tlaxcala, Mexico
Hernandez-Aragon, Laura G.:
Benemerita Univ Autonoma Puebla, Inst Fisiol, Puebla, Mexico
Sanchez-Garcia, Octavio:
Univ Autonoma Tlaxcala, Fac Ciencias Salud, Tlaxcala, Mexico
Martinez-Gomez, Margarita:
Univ Autonoma Tlaxcala, Ctr Tlaxcala Biol Conducta, Tlaxcala, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Celular & Fisiol, Tlaxcala, Mexico
Cuevas-Romero, Estela:
Univ Autonoma Tlaxcala, Ctr Tlaxcala Biol Conducta, Tlaxcala, Mexico
Castelan, Francisco:
Univ Autonoma Tlaxcala, Ctr Tlaxcala Biol Conducta, Tlaxcala, Mexico
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Biol Celular & Fisiol, Tlaxcala, Mexico
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