Structural identification of the iipo-chitin oligosaccharide nodulation signals of Rhizobium loti
Por:
López-Lara I.M., van den Berg J.D.J., Thomas-Oates J.E., Glushka J., Lugtenberg B.J.J., Spaink H.P.
Publicada:
1 ene 1995
Resumen:
Rhizobium loti is a fast-growing Rhizobium species that has been described as a microsymbiont of plants of the genus Lotus. Nodulation studies show that Lotus plants are nodulated by R loti, but not by most other Rhizobium strains, indicating that R. loti produces specific lipo-chitin oligosaccharides (LCOs) which are necessary for the nodulation of Lotus plants. The LCOs produced by five different Rhizobium ioti strains have been purified and were shown to be N-acetylglucosamine pentasaccharides of which the non-reducing residue is N-methylated and N-acylated with c/s-vaccenic acid (C18:1) or stearic acid (C18:O) and carries a carbamoyl group. In one R. loti strain, NZP2037, an additional carbamoyl group is present on the non-reducing terminal residue. The major class of LCO molecules is substituted on the reducing terminal residue with 4-O-acetylfucose. Addition of LCOs to the roots of Lotus plants results in abundant distortion, swelling and branching of the root hairs, whereas spot inoculation leads to the formation of nodule primordia. Copyright © 1995, Wiley Blackwell. All rights reserved
Filiaciones:
López-Lara I.M.:
Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, Leiden, 2333 AL, Netherlands
van den Berg J.D.J.:
Bijvoet Center for Biomolecular Research., Department of Mass Spectrometry, Utrecht University, Sorbonnnelaan 16, Utrecht, 3584 CA, Netherlands
Thomas-Oates J.E.:
Bijvoet Center for Biomolecular Research., Department of Mass Spectrometry, Utrecht University, Sorbonnnelaan 16, Utrecht, 3584 CA, Netherlands
Glushka J.:
Complex Carbohydrate Research Center, 220 Riverbend Road, Athens, Georgia, 30602, United States
Lugtenberg B.J.J.:
Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, Leiden, 2333 AL, Netherlands
Spaink H.P.:
Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, Leiden, 2333 AL, Netherlands
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