Rue and clove microspheres: antiamebic activity, hemocompatibility, and release kinetics
Por:
Mejía-Argueta E.L., Santillán-Benítez J.G., Flores-Merino M., Cervantes-Rebolledo C., Nequiz-Avendaño M.
Publicada:
1 ene 2022
Categoría:
Medicine (miscellaneous)
Resumen:
Objectives: The alginate microspheres were prepared with methanol extract of rue and essential oil of clove with metronidazole evaluating their biological and physicochemical activities. Materials and methods: Microspheres were evaluated with their sphericity, encapsulation efficiency, degradation, and release kinetics with mathematical models. Also, their hemocompatibility and antiamebic activity were studied on hemolytic, proteolytic, and erythrophagocytic activities ofEntamoeba histolytica. Results: Microspheres were obtained with sphericity equal to one, with encapsulation efficiency greater than 50 % (except CEO in 220 nm). Their degradation was recorded between 8 %-12 % in acidic and basic pH. In addition, its release kinetics had a burst constant in almost all the treatments, with the behavior of Korsmeyer-Peppas and Peppas-Sahlin being of pseudofickian behavior. The microspheres were hemocompatible according to the regulations (<10 %) and additionally decreased virulence factors of the amoeba such as hemolytic, proteolytic, erythrophagocytic activities. Conclusions: The microspheres obtained could be a possible treatment against E. histolytica. Still, other toxicity parameters have to be determined (i.e., cytotoxicity, cytocompatibility, biosafety, body weight, survival rate, weight index of main organs, histopathology of main organs, serum biochemical, etc.), as well as physicochemical parameters such as differential scanning calorimetry, Fourier-transform infrared spectroscopy and viscosity. © 2022 Academia Nacional de Medicina. All rights reserved.
Filiaciones:
Mejía-Argueta E.L.:
Autonomous University of the State of Mexico (UAEMex), Toluca, Mexico
Santillán-Benítez J.G.:
Autonomous University of the State of Mexico (UAEMex), Toluca, Mexico
Flores-Merino M.:
Autonomous University of the State of Mexico (UAEMex), Experimental Chemistry Laboratory, Faculty of Chemistry. UAEMex, Toluca, 50120, Mexico
Cervantes-Rebolledo C.:
UICUI., Ixtlahuaca, 50740, Mexico
Nequiz-Avendaño M.:
National Autonomous University of Mexico (UNAM), Experimental Medicine Research Unit, Faculty of Medicine, General Hospital “067720, Mexico
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