Probiotic activity traits in vitro and production of antimicrobial peptides by Lactobacillaceae isolates from pulque using Lactobacillus acidophilus NCFM as control
Por:
Ruiz-Ramirez, Yesica, Cecilia Guadarrama-Mendoza, Paula, Escalante, Adelfo, Giles-Gomez, Martha, Valadez-Blanco, Rogelio
Publicada:
1 jun 2022
Categoría:
Microbiology
Resumen:
The objective of this work was to determine in vitro probiotic activity
traits of 11 lactic acid bacteria (LAB) strains isolated from pulque
obtained from three different locations in the Mexican states of Oaxaca
and Puebla using the probiotic strain Lactobacillus acidophilus NCFM as
a positive control, and to detect their production of antimicrobial
peptides, including bacteriocins and peptidoglycan hydrolases (PGH). The
LAB isolates were identified by sequencing of their 16S rRNA as
belonging to four different genera of the Lactobacillaceae family:
Lactiplantibacillus, Levilactobacillus, Lacticaseibacillus and
Liquorilactobacillus, corresponding to the species plantarum, brevis,
paracasei and ghanensis, respectively. Most of the strains showed
resistance to high acidity (pH 2) and bile salts (0.5%), with survival
rates up to 87 and 92%, respectively. In addition, most of the strains
presented good antimicrobial activity against the foodborne pathogens
Listeria monocytogenes, ECEC and Salmonella Typhi. The strain
Liquorilactobacillus ghanensis RVG6, newly reported in pulque, presented
an outstanding overall performance on the probiotic activity tests. In
terms of their probiotic activity traits assessed in this work, the
strains compared positively with the control L. acidophilus NCFM, which
is a very-well documented probiotic strain. For the antimicrobial
peptide studies, four strains presented bacteriocin-like mediated
antibiosis and six had significant PGH activity, with two strains
presenting outstanding overall antimicrobial peptide production:
Lacticaseibacillus paracasei RVG3 and Levilactobacillus brevis UTMB2.
The probiotic performance of the isolates was mainly dependent on strain
specificity. The results obtained in this work can foster the
revalorization of pulque as a functional natural product.
Filiaciones:
Ruiz-Ramirez, Yesica:
Univ Tecnol Mixteca, Inst Agroind, Carretera Acatlima Km 2-5, Huajuapan De Leon 69000, Oaxaca, Mexico
Univ Autonoma Metropolitana, Dept Biotecnol, Unidad Iztapalapa, Av San Rafael Atlixco 186, Ciudad De Mexico 09340, Mexico
Cecilia Guadarrama-Mendoza, Paula:
Univ Tecnol Mixteca, Inst Agroind, Carretera Acatlima Km 2-5, Huajuapan De Leon 69000, Oaxaca, Mexico
Escalante, Adelfo:
UNAM, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Av Univ 2001, Cuernavaca 62210, Morelos, Mexico
Giles-Gomez, Martha:
UNAM, Fac Quim, Dept Biol, Circuito Exterior S-N,Cd Univ, Ciudad De Mexico 04510, Mexico
Valadez-Blanco, Rogelio:
Univ Tecnol Mixteca, Inst Agroind, Carretera Acatlima Km 2-5, Huajuapan De Leon 69000, Oaxaca, Mexico
|