Large-Size Subunit Catalases Are Chimeric Proteins: A H2O2 Selecting Domain with Catalase Activity Fused to a Hsp31-Derived Domain Conferring Protein Stability and Chaperone Activity
Por:
Hansberg, Wilhelm, Nava-Ramirez, Teresa, Rangel-Silva, Pablo, Diaz-Vilchis, Adelaida, Mendoza-Oliva, Ayde
Publicada:
1 may 2022
Resumen:
Bacterial and fungal large-size subunit catalases (LSCs) are like
small-size subunit catalases (SSCs) but have an additional C-terminal
domain (CT). The catalytic domain is conserved at both primary sequence
and structural levels and its amino acid composition is optimized to
select H2O2 over water. The CT is structurally conserved, has an amino
acid composition similar to very stable proteins, confers high stability
to LSCs, and has independent molecular chaperone activity. While heat
and denaturing agents increased Neurospora crassa catalase-1 (CAT-1)
activity, a CAT-1 version lacking the CT (C63) was no longer activated
by these agents. The addition of catalase-3 (CAT-3) CT to the CAT-1 or
CAT-3 catalase domains prevented their heat denaturation in vitro.
Protein structural alignments indicated CT similarity with members of
the DJ-1/PfpI superfamily and the CT dimers present in LSCs constitute a
new type of symmetric dimer within this superfamily. However, only the
bacterial Hsp31 proteins show sequence similarity to the bacterial and
fungal catalase mobile coil (MC) and are phylogenetically related to
MC_CT sequences. LSCs might have originated by fusion of SSC and Hsp31
encoding genes during early bacterial diversification, conferring at the
same time great stability and molecular chaperone activity to the novel
catalases.
Filiaciones:
Hansberg, Wilhelm:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Biol Celular & Desarrollo, Mexico City 04510, DF, Mexico
Nava-Ramirez, Teresa:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Biol Celular & Desarrollo, Mexico City 04510, DF, Mexico
Rangel-Silva, Pablo:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Biol Celular & Desarrollo, Mexico City 04510, DF, Mexico
Diaz-Vilchis, Adelaida:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Biol Celular & Desarrollo, Mexico City 04510, DF, Mexico
Mendoza-Oliva, Ayde:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Biol Celular & Desarrollo, Mexico City 04510, DF, Mexico
Univ Texas Southwestern Med Ctr Dallas, Ctr Alzheimers & Neurodegenerat Dis, Dallas, TX 75235 USA
gold, Green Published, Gold, Green
|