p-Chloroamphetamine decreases serotonin and induces apoptosis in granulosa cells and follicular atresia in prepubertal female rats
Por:
Gallegos E., Ascona M., Monroy J., Castro-Manrreza M.E., Aragón-Martínez A., Ayala M.E.
Publicada:
1 ene 2022
Categoría:
Toxicology
Resumen:
Amphetamine derivatives negatively impact serotonin (5-HT) production, which triggers apoptosis in different tissues, depending on the receptor they bind. 5-HT in the ovary stimulates estradiol secretion, a survival factor of granulosa cells. The effect of amphetamine derivatives on the serotonergic system of the ovary and follicular development is unknown. Therefore, in this study, we investigated the effects of p-chloroamphetamine (pCA), derived from amphetamines, on estradiol production, follicular development, apoptosis of granulosa cells, and serotonin 5-HT7 receptor (R5-HT7) expression. Female rats (30 days old) were injected with 10 mg/kg of pCA intraperitoneally and were euthanized 48 or 120 h after treatment. The concentration of 5-HT in the hypothalamus decreased at 48 and 120 h after treatment and in the ovary at 120 h. The serum concentration of estradiol decreased at all times studied. Follicular atresia, TUNEL-positive (apoptotic) granulosa cells and Bax expression were elevated by pCA, but none of these effects was associated with R5-HT7 expression. These results suggest that pCA induces the dysregulation of the serotonergic system in the hypothalamus and the ovary, negatively impacting estradiol production and follicular development. © 2022
Filiaciones:
Gallegos E.:
Laboratorio de Pubertad, Unidad Multidisciplinaria de Investigación, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, AP 9-020, Ciudad de MéxicoC.P. 15000, Mexico
Ascona M.:
Laboratorio de Pubertad, Unidad Multidisciplinaria de Investigación, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, AP 9-020, Ciudad de MéxicoC.P. 15000, Mexico
Monroy J.:
Laboratorio de Pubertad, Unidad Multidisciplinaria de Investigación, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, AP 9-020, Ciudad de MéxicoC.P. 15000, Mexico
Castro-Manrreza M.E.:
Laboratorio de Inmunología y Células Madre, Unidad Multidisciplinaria de Investigación, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, AP 9-020, Ciudad de MéxicoC.P. 15000, Mexico
Aragón-Martínez A.:
Laboratorio de Gametos y Desarrollo Tecnológico, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Los Reyes Iztacala, Tlalnepantla, Estado de México, C.P. 09230, Mexico
Ayala M.E.:
Laboratorio de Pubertad, Unidad Multidisciplinaria de Investigación, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, AP 9-020, Ciudad de MéxicoC.P. 15000, Mexico
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