Comparative analysis of striatal [F-18]FDOPA uptake in a partial lesion model of Parkinson's disease in rats: Ratio method versus graphical model
Por:
Avendano-Estrada, Arturo, Verdugo-Diaz, Leticia, Avila-Rodriguez, Miguel A.
Publicada:
1 abr 2022
Categoría:
Cellular and molecular neuroscience
Resumen:
Animal models of Parkinson's disease are useful to evaluate new
treatments and to elucidate the etiology of the disease. Hence, it is
necessary to have methods that allow quantification of their
effectiveness. [F-18]FDOPA-PET (FDOPA-PET) imaging is outstanding for
this purpose because of its capacity to measure changes in the
dopaminergic pathway noninvasively and in vivo. Nevertheless, PET
acquisition and quantification is time-consuming making it necessary to
find faster ways to quantify FDOPA-PET data. This study evaluated Male
Wistar rats by FDOPA, before and after being partially injured with
6-OHDA unilaterally. MicroPET scans with a duration of 120 min were
acquired and Patlak reference plots were created to estimate the influx
constant K-c in the striatum using the full dynamic scan data.
Additionally, simple striatal-to-cerebral ratios (SCR) of short static
acquisitions were computed and compared with the K-c values. Good
correlation (r > 0.70) was obtained between K-c and SCR, acquired
between 80-120 min after FDOPA administration with frames of 10 or 20
min and both methods were able to separate the FDOPA-uptake of healthy
controls from that of the PD model (SCR -28%, K-c -71%). The present
study concludes that K-c and SCR can be trustfully used to discriminate
partially lesioned rats from healthy controls.
Filiaciones:
Avendano-Estrada, Arturo:
Univ Nacl Autonoma Mexico, Fac Med, Div Invest, Unidad Radiofarm Ciclotron, Mexico City, DF, Mexico
Verdugo-Diaz, Leticia:
Univ Nacl Autonoma Mexico, Fac Med, Dept Fisiol, Lab Biomagnetismo, Mexico City, DF, Mexico
Avila-Rodriguez, Miguel A.:
Univ Nacl Autonoma Mexico, Fac Med, Div Invest, Unidad Radiofarm Ciclotron, Mexico City, DF, Mexico
|