Acetylcholinesterase inhibition and antioxidant activity properties of Petiveria alliacea L.


Por: Zavala-Ocampo, Lizeth M., Aguirre-Hernandez, Eva, Lopez-Camacho, Perla Y., Cardenas-Vazquez, Rene, Dorazco-Gonzalez, Alejandro, Basurto-Islas, Gustavo

Publicada: 1 ene 2022
Resumen:
Ethnopharmacological relevance: Petiveria alliacea L. is traditionally used as a folk medical herb in different regions of the world to treat different ailments including those related to the central nervous system. Previous studies have proved that extracts from P. alliacea show improvement in memory and learning process. Aim of the study: To study extracts, fractions, subfractions and isolated compounds from P. alliacea on acetylcholinesterase inhibition and antioxidant activity. Material and methods: Extracts obtained with different polarity solvents and fractions from P. alliacea were evaluated for their inhibitory activity on acetylcholinesterase by Ellman method. This screening allowed the selection of the methanol fraction as the most active and continued a bio-guided study. The compounds identified in methanol fraction were analyzed by high performance liquid chromatography-mass spectrometry (HPLC-MS). Identification of (E)-Tagetone was performed by 1H and 13C NMR spectra. Moreover, the antioxidant activity was evaluated by DPPH and ABTS methods, and the cell viability was assessed by WST-1 method. Results: Two extracts of different polarity were obtained from P. alliacea. The methanol extract and its fraction showed an inhibitory activity on acetylcholinesterase; however, methanol fraction was found to be most potent with 86.5 % AChE inhibition. The methanol fraction also showed antioxidant activity and was not toxic on SH-SY5Y cells. Different compounds including capreoside, narcissin, indane, (-)-isocaryophyllene, (-)-ß-pinene, (E)-tagetone and peonidin 3-O-sambubioside 5-O-glucoside were identified. Conclusion: This is the first report indicating that P. alliacea methanol fraction and its subfractions bear acetylcholinesterase inhibition and antioxidant activity properties. This work establishes the basis for further studies in the development of new therapies for neurodegenerative disorders such as Alzheimer 's disease. © 2022 Elsevier B.V.

Filiaciones:
Zavala-Ocampo, Lizeth M.:
 Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Cuajimalpa, Ciudad de México, Mexico

 Univ Autonoma Metropolitana, Ciencias Biol & Salud, Ciudad De Mexico, Mexico

Aguirre-Hernandez, Eva:
 Laboratorio de Productos Naturales, Universidad Nacional Autónoma de México, Ciudad de México, Mexico

 Univ Nacl Autonoma Mexico, Lab Prod Nat, Ciudad De Mexico, Mexico

Lopez-Camacho, Perla Y.:
 Departamento de Ciencias Naturales, Universidad Autónoma Metropolitana Cuajimalpa, Ciudad de México, Mexico

 Univ Autonoma Metropolitana Cuajimalpa, Dept Ciencias Nat, Ciudad De Mexico, Mexico

Cardenas-Vazquez, Rene:
 Departamento de Biología Cellular, Universidad Nacional Autónoma de México, Ciudad de México, Mexico

 Univ Nacl Autonoma Mexico, Dept Biol Cellular, Ciudad De Mexico, Mexico

Dorazco-Gonzalez, Alejandro:
 Instituto de Química, Universidad Nacional Autónoma de México, Ciudad de México, Mexico

 Univ Nacl Autonoma Mexico, Inst Quim, Ciudad De Mexico, Mexico

Basurto-Islas, Gustavo:
 División de Ciencias e Ingeniería, Universidad de Guanajuato, León Guanajuato, Mexico

 Univ Guanajuato, Div Ciencias & Ingn, Leon Guanajuato, Mexico
ISSN: 03788741
Editorial
ELSEVIER IRELAND LTD, ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND, Irlanda
Tipo de documento: Article
Volumen: 292 Número:
Páginas:
WOS Id: 000806962400008
ID de PubMed: 35358623