Effectiveness of Two Universal Angiosperm Probe Sets Tested In Silico for Caryophyllids Taxa with Emphasis on Cacti Species
Por:
Chincoya, Delil A., Solorzano, Sofia
Publicada:
1 ene 2022
Resumen:
In angiosperms, huge advances in massive DNA sequencing technologies have impacted phylogenetic studies. Probe sets have been developed with the purpose of recovering hundreds of orthologous loci of targeted DNA sequences (TDS) across different plant lineages. We tested in silico the effectiveness of two universal probe sets in the whole available genomes of Caryophyllids, emphasizing phylogenetic issues in cacti species. A total of 870 TDS (517 TDS from Angiosperm v.1 and 353 from Angiosperms353) were individually tested in nine cacti species and Amaranthus hypochondriacus (external group) with =17 Gbp of available DNA data. The effectiveness was measured by the total number of orthologous loci recovered and their length, the percentage of loci discarded by paralogy, and the proportion of informative sites (PIS) in the alignments. The results showed that, on average, Angiosperms353 was better than Angiosperm v.1 for cacti species, since the former obtained an average of 275.6 loci that represent 123,687 bp, 2.48% of paralogous loci, and 4.32% of PIS in alignments, whereas the latter recovered 148.4 loci (37,683 bp), 10.38% of paralogous loci, and 3.49% of PIS. We recommend the use of predesigned universal probe sets for Caryophyllids, since these recover a high number of orthologous loci that resolve phylogenetic relationships. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Filiaciones:
Chincoya, Delil A.:
Laboratorio de Ecología Molecular y Evolución, UBIPRO, FES Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios 1, Los Reyes Iztacala, Estado de México, Tlalnepantla de Baz, 54090, Mexico
Posgrado en Ciencias Biológicas, UNAM, FES Iztacala, Circuito de Posgrados, Ciudad Universitaria, Coyoacán, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Lab Ecol Mol & Evoluc, UBIPRO, FES Iztacala, Ave los Barrios 1, Tlalnepantla De Baz 54090, Estado De Mexic, Mexico
UNAM, FES Iztacala, Posgrad Ciencias Biol, Ciudad Univ, Ciudad De Mexico 04510, Mexico
Solorzano, Sofia:
Laboratorio de Ecología Molecular y Evolución, UBIPRO, FES Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios 1, Los Reyes Iztacala, Estado de México, Tlalnepantla de Baz, 54090, Mexico
Univ Nacl Autonoma Mexico, Lab Ecol Mol & Evoluc, UBIPRO, FES Iztacala, Ave los Barrios 1, Tlalnepantla De Baz 54090, Estado De Mexic, Mexico
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