Lipid metabolism is involved in the association of rotavirus viroplasms with endoplasmic reticulum membranes


Por: Martinez, Jose L., Eichwald, Catherine, Schraner, Elisabeth M., Lopez, Susana, Arias, Carlos F.

Publicada: 1 abr 2022
Categoría: Virology

Resumen:
Rotavirus (RV) replication occurs in cytoplasmic membrane-less, electron-dense inclusions termed viroplasms, composed of viral and cellular elements. These inclusions have been shown to colocalize with components of the lipid droplets (LDs), unique organelles that play an essential role in lipid metabolism. Given the robust LDs-viroplasm association, LDs have been proposed to serve as a scaffold for viroplasm assembly. Interestingly, no evidence has described the participation of lipid metabolism in other RV replication steps. Here, we report that lipid metabolism is essential to maintain the production of the infectious virus through a process independent of viroplasm biogenesis. Disruption of the lipogenesis-lipolysis balance dissociates endoplasmic reticulum mem-branes from viroplasms, suggesting that lipid metabolism is essential for a continuous flux of lipids to allow the association between viroplasms and ER membranes. LDs could also be relevant as lipid reservoirs for membrane synthesis required to form mature infectious rotavirus particles.

Filiaciones:
Martinez, Jose L.:
 Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, Cuernavaca, Mexico

Eichwald, Catherine:
 Institute of Virology, University of Zurich, Zurich, Switzerland

Schraner, Elisabeth M.:
 Institute of Virology, University of Zurich, Zurich, Switzerland

Lopez, Susana:
 Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, Cuernavaca, Mexico

Arias, Carlos F.:
 Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, Cuernavaca, Mexico
ISSN: 00426822





Virology
Editorial
ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA, Estados Unidos America
Tipo de documento: Article
Volumen: 569 Número:
Páginas: 29-36
WOS Id: 000792615300003
ID de PubMed: 35240536

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