An Artemisia cina n-hexane extract reduces the Haemonchus contortus and Teladorsagia circumcincta fecal egg count in naturally infected periparturient goats
Por:
Isabel Higuera-Piedrahita, Rosa, Dolores-Hernandez, Mariana, Alejandro de la-Cruz-Cruz, Hector, Mario Andrade-Montemayor, Hector, Zamilpa, Alejandro, Lopez-Arellano, Raquel, Gonzalez-Garduno, Roberto, Alfredo Cuellar-Ordaz, Jorge, Mendoza-de-Gives, Pedro, Eugenia Lopez-Arellano, Ma
Publicada:
1 abr 2022
Resumen:
The objective of this study was to evaluate an n-hexane extract of
Artemisia cina (Acn-h) as a natural anthelmintic treatment for
periparturient goats naturally infected with the nematodes Haemonchus
contortus and Teladorsagia circumcincta. A total of 200 periparturient
Alpine and Nubian goats were used. Deworming criteria were based on the
following parameters: fecal egg account (epg), ocular mucosa color
(OMC), and body condition (BC). A previous analysis using coprocultures
of the flock revealed the presence of H. contortus (80%) and T.
circumcincta (20%). The Acn-h contained two new compounds identified by
mass spectrometry data as isoguaiacin and norisoguaiacin at 284.14 and
315 m/z. The lethal effects of Acn-h at 0.5, 1, 2, and 4 mg/mL on H.
contortus adult stages were 31.6, 66.5, 81.3, and 86.9%, respectively
(p < 0.05), showing similar efficacy at 2 and 4 mg/mL with albendazole
(positive control group). Then, two experimental groups, with 100 goats
in peripartum in each, were distributed randomly and treated at day 0 as
follows: group 1 = 4 mg/kg of Acn-h as single oral dose, and group 2 =
control group, treated with water (as a placebo). The epg, OMC, and BC
parameters were recorded at 0 (periparturient period), 7 (birth period),
and 23 (postpartum) days and analyzed using a completely randomized
design with Duncan's test for comparison of means and analysis of
variance. The following epg reductions were recorded in the
Acn-h-treated group as follows: 20.1 +/- 34.4 and 31.7 +/- 38.2% at
days 7 and 23 compared to the control group. During the whole
experiment, no significant differences in OMC or BC were observed in
relation to the control group, excepting at day 23 (p < 0.05) for BC in
the group treated with A. cina. Thus, Acn-h can be a useful natural
alternative tool for the control of the nematodes H. contortus and T.
circumcincta in periparturient goat flocks.
Filiaciones:
Isabel Higuera-Piedrahita, Rosa:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Carretera Cuautitlan Teoloyucan Km 2-5, San Sebastian Xhala 54714, Cuautitlan, Mexico
Dolores-Hernandez, Mariana:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Carretera Cuautitlan Teoloyucan Km 2-5, San Sebastian Xhala 54714, Cuautitlan, Mexico
Alejandro de la-Cruz-Cruz, Hector:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Carretera Cuautitlan Teoloyucan Km 2-5, San Sebastian Xhala 54714, Cuautitlan, Mexico
Mario Andrade-Montemayor, Hector:
Univ Autonoma Queretaro, Granja La Negrita, Av Ciencias S-N, Queretaro 76230, Mexico
Univ Autonoma Queretaro, Fac Ciencias Nat, Av Ciencias S-N, Queretaro 76230, Mexico
Zamilpa, Alejandro:
Inst Mexicano Seguro Social, Ctr Invest Biomed Sur, Argentina 1, Xochitepec 62790, Morelos, Mexico
Lopez-Arellano, Raquel:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Carretera Cuautitlan Teoloyucan Km 2-5, San Sebastian Xhala 54714, Cuautitlan, Mexico
Gonzalez-Garduno, Roberto:
Univ Autonoma Chapingo, Unidad Reg Univ Sursureste, Km 7-5 Carretera Teapa Vicente Guerrero, Teapa 86800, Tabasco, Mexico
Alfredo Cuellar-Ordaz, Jorge:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Carretera Cuautitlan Teoloyucan Km 2-5, San Sebastian Xhala 54714, Cuautitlan, Mexico
Mendoza-de-Gives, Pedro:
Inst Nacl Invest Forestales Agr & Pecuarias, Ctr Nacl Invest Disciplinaria Salud Anim & Inocui, Carr Fed Cuernavaca Cuautla 8534 Col Progreso, Jiutepec 62550, Morelos, Mexico
Eugenia Lopez-Arellano, Ma:
Inst Nacl Invest Forestales Agr & Pecuarias, Ctr Nacl Invest Disciplinaria Salud Anim & Inocui, Carr Fed Cuernavaca Cuautla 8534 Col Progreso, Jiutepec 62550, Morelos, Mexico
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