MRX8, the conserved mitochondrial YihA GTPase family member, is required for de novo Cox1 synthesis at suboptimal temperatures in Saccharomyces cerevisiae
Por:
Verma, Yash, Mehra, Upasana, Pandey, Dharmendra Kumar, Kar, Joy, Perez-Martinez, Xochitl, Jana, Siddhartha S., Datta, Kaustuv
Publicada:
1 nov 2021
Resumen:
The synthesis of Cox1, the conserved catalytic-core subunit of Complex
IV, a multisubunit machinery of the mitochondria! oxidative
phosphorylation (OXPHOS) system under environmental stress, has not been
sufficiently addressed. In this study, we show that the putative YihA
superfamily GTPase, Mrx8, is a bona fide mitochondria! protein required
for Cox1 translation initiation and elongation during suboptimal growth
condition at 16 degrees C. Mrx8 was found in a complex with
mitochondrial ribosomes, consistent with a role in protein synthesis.
Cells expressing mutant Mrx8 predicted to be defective in guanine
nucleotide binding and hydrolysis were compromised for robust cellular
respiration. We show that the requirement of Pet309 and Mss51 for
cellular respiration is not bypassed by overexpression of Mrx8 and vice
versa. Consistently the ribosomal association of Mss51 is independent of
Mrx8. Significantly, we find that GTPBP8, the human orthologue,
complements the loss of cellular respiration in Amrx8 cells and GTPBP8
localizes to the mitochondria in mammalian cells. This strongly suggests
a universal role of the MRX8 family of proteins in regulating
mitochondrial function.
Filiaciones:
Verma, Yash:
Univ Delhi, Dept Genet, South Campus, New Delhi 110021, India
Mehra, Upasana:
Univ Delhi, Dept Genet, South Campus, New Delhi 110021, India
Pandey, Dharmendra Kumar:
Univ Delhi, Dept Genet, South Campus, New Delhi 110021, India
Kar, Joy:
Indian Assoc Cultivat Sci, Sch Biol Sci, Kolkata 700032, India
Perez-Martinez, Xochitl:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Genet Mol, Mexico City 04510, DF, Mexico
Jana, Siddhartha S.:
Indian Assoc Cultivat Sci, Sch Biol Sci, Kolkata 700032, India
Datta, Kaustuv:
Univ Delhi, Dept Genet, South Campus, New Delhi 110021, India
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