Complete Genome Sequencing of a Novel Gloeobacter Species from a Waterfall Cave in Mexico
Por:
Saw, Jimmy H., Cardona, Tanai, Montejano, Gustavo
Publicada:
1 ene 2021
Resumen:
Only two complete genomes of the cyanobacterial genus Gloeobacter from two very different regions of the world currently exist. Here, we present the complete genome sequence of a third member of the genus isolated from a waterfall cave in Mexico. Analysis of the average nucleotide identities (ANIs) between published Gloeobacter genomes revealed that the complete genome of this new member is only 92.7% similar to Gloeobacter violaceus and therefore we determined it to be a new species. We propose to name this new species Gloeobacter morelensis after the location in Mexico where it was isolated. The complete genome consists of one circular chromosome (4,921,229 bp), one linear plasmid (172,328 bp), and one circular plasmid (8,839 bp). Its genome is the largest of all completely sequenced genomes of Gloeobacter species. Pangenomic comparisons revealed that G. morelensis encodes 759 genes not shared with other Gloeobacter species. Despite being more closely related to G. violaceus, it features an extremely divergent psbA gene encoding an atypical D1 core subunit of Photosystem II previously only found within the genome of Gloeobacter kilaueensis. In addition, we detected evidence of concerted evolution of psbA genes encoding identical D1 in all three Gloeobacter genomes, a characteristic that seems widespread in cyanobacteria and may therefore be traced back to their last common ancestor. © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
Filiaciones:
Saw, Jimmy H.:
Department of Biological Sciences, George Washington UniversityDC, United States
George Washington Univ, Dept Biol Sci, Washington, DC 20052 USA
Cardona, Tanai:
Department of Life Sciences, Imperial College London, United Kingdom
Imperial Coll London, Dept Life Sci, London, England
Montejano, Gustavo:
Laboratorio de Ficología, National Autonomous University of Mexico, Facultad de Ciencias, Ciudad de México, Mexico
Univ Nacl Autonoma Mexico, Fac Ciencias, Lab Ficol, Ciudad De Mexico, Mexico
Green Published, gold, Gold, Green
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