Effect of exposure to low doses of ozone on interleukin 17A expression during progressive neurodegeneration in the rat hippocampus
Por:
Solleiro-Villavicencio, H., Hernandez-Orozco, E., Rivas-Arancibia, S.
Publicada:
1 nov 2021
Ahead of Print:
1 ene 2018
Resumen:
Introduction: Chronic exposure to low doses of ozone causes oxidative
stress and loss of regulation of the inflammatory response, leading to
progressive neurodegeneration.
Objective: We studied the effect of chronic exposure to low doses of
ozone on IL-17A concentration and expression in neurons, nnicroglia,
astrocytes, and T cells in the rat hippocampus.
Methods: We used 72 Wistar rats, divided into 6 groups (n = 12): a
control group (no ozone exposure) and 5 groups exposed to ozone (0.25
ppm, 4 h daily) for 7, 15, 30, 60, and 90 days. We processed 6 rats from
each group to quantify IL-17A by ELISA; the remaining 6 were processed
for immunohistochemistry (against IL-17A and GFAP, Iba1, NeuN, and CD3).
Results: The ELISA study data showed a significant increase in IL-17A
concentrations in the 7-, 15-, 30-, and 60-day exposure groups, with
regard to the control group (P< .05). Furthermore, they indicate that
hippocampal neurons were the cells showing greatest immunoreactivity
against IL-17A between 60 and 90 days of exposure to ozone; we also
observed an increase in activated astrocytes in the 30- and 60-day
exposure groups.
Conclusion: Exposure to ozone in rats induces an increase in IL-17A
expression, mainly in hippocampal neurons, accompanied by hippocampal
astrocyte activation during chronic neurodegeneration, similar to that
observed in Alzheimer disease in humans. (C) 2018 Sociedad Espanola de
Neurologia. Published by Elsevier Espana, S. L. U.
Filiaciones:
Solleiro-Villavicencio, H.:
Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Hernandez-Orozco, E.:
Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Rivas-Arancibia, S.:
Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
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