A novel model to culture cells from giant cell tumor of bone using three-dimensional (3D) polycaprolactone scaffold
Por:
Estrada-Villasenor, Erendira, Valdes-Flores, Margarita, Meneses-Garcia, Abelardo, Silva-Bermudez, Phaedra, Pichardo-Bahena, Raul, Ostoa-Saloma, Pedro, Mercado-Celis, Gabriela, Delgado-Cedillo, Ernesto D., Olivos-Meza, Anell, Landa-Solis, Carlos
Publicada:
1 sep 2021
Ahead of Print:
1 jun 2021
Resumen:
Two-dimensional (2D) culture of cells from giant cell tumor of bone
(GCTB) is affected by loss of the multinucleated giant cells in
subsequent passages. Therefore, there is limited time to study GCTB with
all its histological components in 2D culture. Here, we explored the
possibility of culturing GCTB cells on a polycaprolactone (PCL)-printed
scaffold. We also evaluated the viability of the cultured cells and
their adherence to the PCL scaffold at day 14 days using
immunofluorescence analysis with calcein, vinculin, and phalloidin.
Using the histological technique with hematoxylin and eosin staining, we
observed all the histological components of GCTB in this 3D model.
Immunohistochemical assays with cathepsin K, p63, and receptor activator
of nuclear factor (NF)-kappa B ligand (RANKL) yielded positive results
in this construct, which allowed us to confirm that the seeded cells
maintained the expression of GCTB markers. Based on these findings, we
concluded that the PCL scaffold is an efficient model to culture GCTB
cells, and the cell viability and adherence to the scaffold can be
preserved for up to 14 days. Moreover, this model can also be used in
subsequent studies to assess in vitro cell-cell interactions and
antineoplastic efficacy of certain agents to establish a treatment
against GCTB.
Filiaciones:
Estrada-Villasenor, Erendira:
Pathology Service, National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra, México City, Mexico
Valdes-Flores, Margarita:
Genetics Laboratory, National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra, Mexico City, Mexico
Meneses-Garcia, Abelardo:
Pathology Service, National Cancer Institute, Mexico City, Mexico
Silva-Bermudez, Phaedra:
Tissue Engineering and Cell Therapy Unit, National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra, Mexico City, Mexico
Pichardo-Bahena, Raul:
Pathology Service, National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra, México City, Mexico
Ostoa-Saloma, Pedro:
Department of Immunology, Institute of Biomedical Research, National Autonomous University of Mexico, Mexico City, Mexico
Mercado-Celis, Gabriela:
Laboratory of Clinical Genomics, Division of Graduate Studies and Research, Faculty of Odontology, National Autonomous University of Mexico, Mexico City, Mexico
Delgado-Cedillo, Ernesto D.:
Bone Tumors Service, National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra, Mexico City, Mexico
Olivos-Meza, Anell:
Sports Orthopedics and Arthroscopy, National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra, Mexico City, Mexico
Landa-Solis, Carlos:
Tissue Engineering and Cell Therapy Unit, National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra, Mexico City, Mexico
gold, Green Accepted, Gold, Green
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