Effects on capsicum annuum plants colonized with trichoderma atroviride P. Karst strains genetically modified in taswo1, a gene coding for a protein with expansin-like activity
Por:
Sanchez-Cruz, Ricardo, Mehta, Richa, Atriztan-Hernandez, Karina, Martinez-Villamil, Olivia, Sánchez-Carbente M.R., Sanchez-Reyes, Ayixon, Lira-Ruan, Veronica, Gonzalez-Chavez, Carlos Alberto, Tabche-Barrera, Maria Luisa, Barcenas-Rodriguez, Roberto Carlos, Batista-Garcia, Ramon Alberto, Herrera-Estrella, Alfredo, Balcazar-Lopez, Edgar, Folch-Mallol, Jorge Luis
Publicada:
1 ene 2021
Resumen:
Here, we analyzed the effects on Capsicum annuum plants of Trichoderma atroviride P. Karst strains altered in the expression of SWOLLENIN (SWO1), a protein with amorphogenic activity on plant cell wall components. Strains of T. atroviride that overexpressed the Taswo1 gene were constructed as well as deletion mutants. A novel, cheap and accurate method for assessing root colonization was developed. Colonization assays showed that the Taswo1 overexpressing strains invaded the host root better than the WT, resulting in a stronger plant growth-promoting effect. The expression of plant defense marker genes for both the systemic acquired resistance and induced systemic resistance pathways was enhanced in plants inoculated with Taswo1 overexpressing strains, while inoculation with deletion mutant strains resulted in a similar level of expression to that observed upon inoculation with the wild-type strain. Response to pathogen infection was also enhanced in the plants inoculated with the Taswo1 overexpressing strains, and surprisingly, an intermediate level of protection was achieved with the mutant strains. Tolerance to abiotic stresses was also higher in plants inoculated with the Taswo1 overexpressing strains but was similar in plants inoculated with the wild-type or the mutant strains. Compatible osmolyte production in drought conditions was studied. This study may contribute to improving Trichoderma biocontrol and biofertilization abilities. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Filiaciones:
Sanchez-Cruz, Ricardo:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, 62209, Mexico
Mehta, Richa:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, 62209, Mexico
Atriztan-Hernandez, Karina:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, 62209, Mexico
Laboratorio Nacional de Genómica para la Biodiversidad, Cinvestav Campus Guanajuato, Km. 9.6 Libramiento Norte, Carretera Irapuato León, Irapuato, Gto, 36824, Mexico
Martinez-Villamil, Olivia:
Facultad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos Cuernavaca, Cuernavaca, Morelos, 62209, Mexico
Sánchez-Carbente M.R.:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, 62209, Mexico
Sanchez-Reyes, Ayixon:
Cátedras CONACyT-Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62209, Mexico
Lira-Ruan, Veronica:
Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, 62209, Mexico
Gonzalez-Chavez, Carlos Alberto:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62209, Mexico
Tabche-Barrera, Maria Luisa:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62209, Mexico
Barcenas-Rodriguez, Roberto Carlos:
Departamento de Ingeniería Bioquímica, Cinvestav Irapuato, Libramiento Norte Carretera, Irapuato León Kilómetro 9.6, Carretera Irapuato León, Irapuato, Gto, 36821, Mexico
Batista-Garcia, Ramon Alberto:
Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, 62209, Mexico
Herrera-Estrella, Alfredo:
Laboratorio Nacional de Genómica para la Biodiversidad, Cinvestav Campus Guanajuato, Km. 9.6 Libramiento Norte, Carretera Irapuato León, Irapuato, Gto, 36824, Mexico
Balcazar-Lopez, Edgar:
Centro Universitario de Ciencias Exactas e Ingeniería, Departamento de Farmacobiología, Universidad de Guadalajara, García Barragán # 1451, Guadalajara, Jalisco, 44430, Mexico
Folch-Mallol, Jorge Luis:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, 62209, Mexico
gold, Green Published, Gold
|