Bacterial Quorum-Sensing Signaling-Related drr1 Mutant Influences Abscisic Acid Responsiveness in Arabidopsis thaliana L.
Por:
Barrera-Ortiz, Salvador, Lopez-Garcia, Claudia Marina, Ortiz-Castro, Randy, Guevara-Garcia, Angel Arturo, Lopez-Bucio, Jose
Publicada:
1 ene 2022
Ahead of Print:
1 feb 2021
Resumen:
N-acyl-L-homoserine lactones (AHLs) are involved in cell-to-cell
communication in Gram-negative bacteria through a process termed
quorum-sensing (QS). In this report, we evaluated the response of
Arabidopsis thaliana primary roots to abscisic acid (ABA) in wild-type
(WT) and decanamide resistant root 1 (drr1) mutant, previously reported
to be resistant to N-decanoyl-L-homoserine lactone (C10-HL). When
compared to WT seedlings, drr1 mutants were hypersensitive to ABA and
had primary roots shorter, which correlated with lower cell division in
meristems, a higher concentration of endogenous ABA, and a greater
expression of ABI5 gene; and this shortened primary root phenotype was
reversed in drr1abi5 double mutants. An analysis of expression of
ABSCISIC ACID INSENSITIVE 4 (ABI4) showed an ABA-inducible pattern in
primary root tips, which was further increased in drr1 mutant seedlings.
Comparison of seed germination in WT, drr1, abi5, and drr1abi5 lines
showed higher germination percentages in the following order under
control condition: abi5 > drr1abi5 > WT > drr1, while abi5 and drr1abi5
germinated faster and drr1 germinated slower with respect to WT under
ABA condition. Taken together, our results suggest that DRR1 is a
negative regulator of ABA signaling probably acting upstream of the
transcription factors ABI4 and ABI5, which influence ABA responsiveness
in primary roots and seed germination.
Filiaciones:
Barrera-Ortiz, Salvador:
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico
Lopez-Garcia, Claudia Marina:
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico
Ortiz-Castro, Randy:
CONACYT-Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera antigua a Coatepec 351, Colonia El Haya, Xalapa, Veracruz CP 91070, Mexico
Guevara-Garcia, Angel Arturo:
Instituto de Biotecnología, Departamento de Biología Molecular de Plantas, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico
Lopez-Bucio, Jose:
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, Morelia, Michoacán C. P. 58030, Mexico
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