|Isolation and characterization of novel bacteriophages as a potential therapeutic option for Escherichia coli urinary tract infections
Por:
Gonzalez-Villalobos, Edgar, Maria Ribas-Aparicio, Rosa, Rodea Montealegre, Gerardo Erbey, Belmont-Monroy, Laura, Ortega-Garcia, Yerisaidy, Aparicio-Ozores, Gerardo, Luis Balcazar, Jose, Alberto Eslava-Campos, Carlos, Hernandez-Chinas, Ulises, Molina-Lopez, Jose
Publicada:
1 jul 2021
Ahead of Print:
1 jul 2021
Resumen:
Urinary tract infections (UTIs) are mainly caused by uropathogenic
Escherichia coli (UPEC), whose impact can be exacerbated by
multidrug-resistant (MDR) strains. Effective control strategies are,
therefore, urgently needed. Among them, phage therapy represents a
suitable alternative. Here, we describe the isolation and
characterization of novel phages from wastewater samples, as well as
their lytic activity against biofilm and adherence of UPEC to HEp-2
cells. The results demonstrated that phage vB_EcoM-phiEc1 (phi Ec1)
belongs to Myoviridae family, whereas vB_EcoS-phiEc3 (phi Ec3) and
vB_EcoS-phiEc4 (phi Ec4) belong to Siphoviridae family. Phages showed
lytic activity against UPEC and gut commensal strains. Phage phi Ec1
lysed UPEC serogroups, whereas phages phi Ec3 and phi Ec4 lysed only UTI
strains with higher prevalence toward the O25 serogroup. Moreover,
phages phi Ec1 and phi Ec3 decreased both biofilm formation and
adherence, whereas phi Ec4 was able to decrease adherence but not
biofilm formation. In conclusion, these novel phages showed the ability
to decrease biofilm and bacterial adherence, making them promising
candidates for effective adjuvant treatment against UTIs caused by MDR
UPEC strains.
Filiaciones:
Gonzalez-Villalobos, Edgar:
Inst Politecn Nacl IPN, Dept Microbiol, Escuela Nacl Ciencias Biol ENCB, Prolongac De Carpio & Plan De Ayala S-N, Mexico City 11340, DF, Mexico
Univ Nacl Autonoma Mexico, Unidad Perifer Invest Bas & Clin Enfermedades Inf, Fac Med, Dept Salud Publ,Div Invest, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Lab Patogenicidad Bacteriana, Unidad Hematooncol & Invest, Hosp Infantil Mexico Federico Gomez,Fac Med, Dr Marquez 162, Mexico City 06720, DF, Mexico
Catalan Inst Water Res ICRA, Girona 17003, Spain
Maria Ribas-Aparicio, Rosa:
Inst Politecn Nacl IPN, Dept Microbiol, Escuela Nacl Ciencias Biol ENCB, Prolongac De Carpio & Plan De Ayala S-N, Mexico City 11340, DF, Mexico
Rodea Montealegre, Gerardo Erbey:
Univ Nacl Autonoma Mexico, Unidad Perifer Invest Bas & Clin Enfermedades Inf, Fac Med, Dept Salud Publ,Div Invest, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Lab Patogenicidad Bacteriana, Unidad Hematooncol & Invest, Hosp Infantil Mexico Federico Gomez,Fac Med, Dr Marquez 162, Mexico City 06720, DF, Mexico
Belmont-Monroy, Laura:
Inst Politecn Nacl IPN, Dept Microbiol, Escuela Nacl Ciencias Biol ENCB, Prolongac De Carpio & Plan De Ayala S-N, Mexico City 11340, DF, Mexico
Univ Nacl Autonoma Mexico, Unidad Perifer Invest Bas & Clin Enfermedades Inf, Fac Med, Dept Salud Publ,Div Invest, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Lab Patogenicidad Bacteriana, Unidad Hematooncol & Invest, Hosp Infantil Mexico Federico Gomez,Fac Med, Dr Marquez 162, Mexico City 06720, DF, Mexico
Ortega-Garcia, Yerisaidy:
Inst Politecn Nacl IPN, Dept Microbiol, Escuela Nacl Ciencias Biol ENCB, Prolongac De Carpio & Plan De Ayala S-N, Mexico City 11340, DF, Mexico
Univ Nacl Autonoma Mexico, Unidad Perifer Invest Bas & Clin Enfermedades Inf, Fac Med, Dept Salud Publ,Div Invest, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Lab Patogenicidad Bacteriana, Unidad Hematooncol & Invest, Hosp Infantil Mexico Federico Gomez,Fac Med, Dr Marquez 162, Mexico City 06720, DF, Mexico
Aparicio-Ozores, Gerardo:
Inst Politecn Nacl IPN, Dept Microbiol, Escuela Nacl Ciencias Biol ENCB, Prolongac De Carpio & Plan De Ayala S-N, Mexico City 11340, DF, Mexico
Luis Balcazar, Jose:
Catalan Inst Water Res ICRA, Girona 17003, Spain
Univ Girona, Girona 17004, Spain
Alberto Eslava-Campos, Carlos:
Univ Nacl Autonoma Mexico, Unidad Perifer Invest Bas & Clin Enfermedades Inf, Fac Med, Dept Salud Publ,Div Invest, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Lab Patogenicidad Bacteriana, Unidad Hematooncol & Invest, Hosp Infantil Mexico Federico Gomez,Fac Med, Dr Marquez 162, Mexico City 06720, DF, Mexico
Hernandez-Chinas, Ulises:
Univ Nacl Autonoma Mexico, Unidad Perifer Invest Bas & Clin Enfermedades Inf, Fac Med, Dept Salud Publ,Div Invest, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Lab Patogenicidad Bacteriana, Unidad Hematooncol & Invest, Hosp Infantil Mexico Federico Gomez,Fac Med, Dr Marquez 162, Mexico City 06720, DF, Mexico
Molina-Lopez, Jose:
Univ Nacl Autonoma Mexico, Unidad Perifer Invest Bas & Clin Enfermedades Inf, Fac Med, Dept Salud Publ,Div Invest, Mexico City 04510, DF, Mexico
Univ Nacl Autonoma Mexico, Lab Patogenicidad Bacteriana, Unidad Hematooncol & Invest, Hosp Infantil Mexico Federico Gomez,Fac Med, Dr Marquez 162, Mexico City 06720, DF, Mexico
hybrid, Green Published, Hybrid Gold, Green
|