Assessing the treatment of cannabidiolic acid methyl ester: a stable synthetic analogue of cannabidiolic acid on c-Fos and NeuN expression in the hypothalamus of rats
Por:
Murillo-Rodriguez, Eric, Millan-Aldaco, Diana, Arankowsky-Sandoval, Gloria, Yamamoto, Tetsuya, Pertwee, Roger G., Parker, Linda, Mechoulam, Raphael
Publicada:
12 jul 2021
Resumen:
Background Cannabidiol (CBD), the non-psychotropic compound from
Cannabis sativa, shows positive results on controlling several health
disturbances; however, comparable data regarding additional chemical
from C. sativa, such as cannabidiolic acid (CBDA), is scarce due to its
instability. To address this limitation, a stable CBDA analogue, CBDA
methyl ester (HU-580), was synthetized and showed CBDA-like effects.
Recently, we described that HU-580 increased wakefulness and
wake-related neurochemicals. Objective To extend the comprehension of
HU-580 ` s properties on waking, the c-Fos and NeuN expression in a
wake-linked brain area, the hypothalamus was evaluated. Methods c-Fos
and NeuN expression in hypothalamic sections were analyzed after the
injections of HU-580 (0.1 or 100 mu g/kg, i.p.). Results Systemic
administrations of HU-580 increased c-Fos and neuronal nuclei (NeuN)
expression in hypothalamic nuclei, including the dorsomedial
hypothalamic nucleus dorsal part, dorsomedial hypothalamic nucleus
compact part, and dorsomedial hypothalamic nucleus ventral part.
Conclusion HU-580 increased c-Fos and NeuN immunoreactivity in
hypothalamus nuclei suggesting that this drug might modulate the
sleep-wake cycle by engaging the hypothalamus.
Filiaciones:
Murillo-Rodriguez, Eric:
Univ Anahuac Mayab Merida, Div Ciencias Salud, Escuela Med, Lab Neurociencias Mol & Integrat, Km 15-5 Carretera Merida Progreso, Merida 97308, Yucatan, Mexico
Intercontinental Neurosci Res Grp, Merida, Yucatan, Mexico
Millan-Aldaco, Diana:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurociencia Cognit, Div Neurociencias, Ciudad De Mexico, Mexico
Arankowsky-Sandoval, Gloria:
Univ Autonoma Yucatan, Ctr Invest Reg Dr Hideyo Noguchi, Merida, Yucatan, Mexico
Yamamoto, Tetsuya:
Intercontinental Neurosci Res Grp, Merida, Yucatan, Mexico
Tokushima Univ, Grad Sch Technol Ind & Social Sci, Tokushima, Japan
Pertwee, Roger G.:
Univ Aberdeen, Inst Med Sci, Sch Med Med Sci & Nutr, Aberdeen, Scotland
Parker, Linda:
Univ Guelph, Dept Psychol, Guelph, ON, Canada
Univ Guelph, Neurosci Grad Program, Guelph, ON, Canada
Mechoulam, Raphael:
Hebrew Univ Jerusalem, Med Fac, Inst Drug Res, Jerusalem, Israel
Green Published, gold, Gold, Green
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