Sex Dimorphic Changes in Trh Gene Methylation and Thyroid-Axis Response to Energy Demands in Maternally Separated Rats
Por:
Jaimes-Hoy, Lorraine, Perez-Maldonado, Adrian, Narvaez Bahena, Elian, de la Cruz Guarneros, Natalia, Rodriguez-Rodriguez, Adair, Charli, Jean-Louis, Soberon, Xavier, Joseph-Bravo, Patricia
Publicada:
1 ago 2021
Categoría:
Endocrinology
Resumen:
The hypothalamus-pituitary-thyroid (HPT) axis regulates energy balance
through the pleiotropic action of thyroid hormones. HPT basal activity
and stimulation by cold or voluntary exercise are repressed by previous
chronic stress in adults. Maternal separation (MS) modifies HPT basal
activity; we thus studied the response of the axis to energy demands and
analyzed possible epigenetic changes on Trh promoter. Nonhandled (NH) or
MS male Wistar rats were cold exposed 1 h at adulthood; Trh expression
in the hypothalamic paraventricular nucleus (PVN) and serum thyrotropin
(TSH) concentration were increased only in NH rats.Two weeks of
voluntary exercise decreased fat mass and increased Trh expression, and
thyroid hormones concentration changed proportionally to running
distance in NH male rats and MS male rats. Although NH females ran more
than MS and much more than males, exercise decreased body weight and fat
mass only in NH rats with no change on any parameter of the HPT axis but
increased Pomc expression in arcuate-nucleus of NH and Npy in MS
females. Overall, the methylation pattern of PVN Trh gene promoter was
similar in NH males and females; MS modified methylation of specific CpG
sites, a thyroid hormone receptor (THR)-binding site present after the
initiation site was hypomethylated in MS males; in MS females, the THR
binding site of the proximal promoter (site 4) and 2 sites in the first
intron were hypermethylated. Our studies showed that, in a sex-dimorphic
manner, MS blunted the responses of HPT axis to energy demands in adult
animals and caused methylation changes on Trh promoter that could
alterT3 feedback.
Filiaciones:
Jaimes-Hoy, Lorraine:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Cuernavaca, Morelos, Mexico
Perez-Maldonado, Adrian:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Cuernavaca, Morelos, Mexico
Narvaez Bahena, Elian:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Cuernavaca, Morelos, Mexico
de la Cruz Guarneros, Natalia:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Cuernavaca, Morelos, Mexico
Rodriguez-Rodriguez, Adair:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Cuernavaca, Morelos, Mexico
Charli, Jean-Louis:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Cuernavaca, Morelos, Mexico
Soberon, Xavier:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca, Morelos, Mexico
Inst Nacl Med Genom, Mexico City, DF, Mexico
Joseph-Bravo, Patricia:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Genet Desarrollo & Fisiol Mol, Cuernavaca, Morelos, Mexico
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