Pseudomonas aeruginosa induces spatio-temporal secretion of IL-1 beta, TNF alpha, proMMP-9, and reduction of epithelial E-cadherin in human alveolar epithelial type II (A549) cells
Por:
Fuentes-Zacarias, Paulina, Armando Arzate-Castaneda, Diego, Sosa-Gonzalez, Irma, Villeda-Gabriel, Graciela, Morales-Mendez, Iyari, Osorio-Caballero, Mauricio, Cecilia Helguera-Repetto, Addy, Nestor Diaz, Fabian, Garcia-Lopez, Guadalupe, Flores-Herrera, Oscar, Arenas-Huertero, Francisco, Eslava-Campos, Carlos, Diaz-Ruiz, Oscar, Flores-Herrera, Hector
Publicada:
1 ene 2021
Categoría:
Biochemistry, genetics and molecular biology (miscellaneous)
Resumen:
Pseudomonas aeruginosa, is an opportunistic bacterium with a high
prevalence in diverse pulmonary infections. Although several genes are
involved in the system of resistance and evasion of the immunological
response of the host, little is known about the inflammatory,
degradative, and cell-binding response induced by P. aeruginosa in human
lung alveolar epithelial cells. The purpose of this study was to
determine the cytokine expression (IL-1 beta and TNF alpha), pro matrix
metalloproteinases activation (proMMP-2 and proMMP-9), and the effects
on the cell-binding adhesion protein (E-cadherin) in an in vitro model
of human lung alveolar epithelial cells. A549 cells were stimulated with
a different number of colony-forming units of P. aeruginosa for 3, 6,
and 24 hours. Subsequently, the culture medium was collected, IL-1 beta
and TNF alpha levels were evaluated by ELISA; proMMP-2 and -9 levels
were determined by substrate gel zymography, and the MMP-9 and
E-cadherin assessed by immunostaining of A549 cells. Our results
demonstrated that P. aeruginosa induces mainly the secretion of TNF
alpha, increases act MMP-9 level, and significantly reduces the level of
E-cadherin in the A549 cells. In summary, the inflammatory/degradative
process induced by P. aeruginosa modulates the expression of the
E-cadherin protein. The probable clinical implications of this study
suggest the use of inhibitors that reduce the degradative activity of
proMMP-9 which will be further explored in the next phase of this study.
Filiaciones:
Fuentes-Zacarias, Paulina:
Inst Nacl Perinatol Isidro Espinoza Reyes INPerIE, Dept Inmunobioquim, Ciudad De Mexico, Mexico
Armando Arzate-Castaneda, Diego:
Inst Nacl Perinatol Isidro Espinoza Reyes INPerIE, Dept Inmunobioquim, Ciudad De Mexico, Mexico
Sosa-Gonzalez, Irma:
INPerIER, Dept Inmunol & Infectol, Ciudad De Mexico, Mexico
Villeda-Gabriel, Graciela:
INPerIER, Dept Inmunol & Infectol, Ciudad De Mexico, Mexico
Morales-Mendez, Iyari:
Inst Nacl Perinatol Isidro Espinoza Reyes INPerIE, Dept Inmunobioquim, Ciudad De Mexico, Mexico
Osorio-Caballero, Mauricio:
INPerIER, Dept Salud Sexual & Reprod, Ciudad De Mexico, Mexico
Cecilia Helguera-Repetto, Addy:
Inst Nacl Perinatol Isidro Espinoza Reyes INPerIE, Dept Inmunobioquim, Ciudad De Mexico, Mexico
Nestor Diaz, Fabian:
INPerIER, Dept Fisiol & Desarrollo Celular, Ciudad De Mexico, Mexico
Garcia-Lopez, Guadalupe:
INPerIER, Dept Fisiol & Desarrollo Celular, Ciudad De Mexico, Mexico
Flores-Herrera, Oscar:
UNAM, Dept Bioquim, Escuela Med, Ciudad De Mexico, Mexico
Arenas-Huertero, Francisco:
Hosp Infantil Mexico Dr Federico Gomez, Dept Patol Expt, Ciudad De Mexico, Mexico
Eslava-Campos, Carlos:
Hosp Infantil Mexico Dr Federico Gomez, Lab Patogenicidad Bacteriana, Unidad Hematooncol & Invest, Ciudad De Mexico, Mexico
Diaz-Ruiz, Oscar:
Emory Univ, Dept Pharmacol, Sch Med, Atlanta, GA 30322 USA
Flores-Herrera, Hector:
Inst Nacl Perinatol Isidro Espinoza Reyes INPerIE, Dept Inmunobioquim, Ciudad De Mexico, Mexico
Hybrid Gold, Gold
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