Roles of culture media and oxygen transfer in the scale-up from shake flasks to pneumatic bioreactor of the plant growth-promoting bacterium Rhizobium phaseoli
Por:
Garcia-Cabrera, I, R., Valdez-Cruz, N. A., Daniel-Vazquez, A., Blancas-Cabrera, A., Trujillo-Rollan, M. A.
Publicada:
1 may 2021
Categoría:
Chemical engineering (miscellaneous)
Resumen:
The success of a Rhizobium sp. inoculant depends on a product with a
high number of live bacteria that can nodulate a leguminous plant. With
the idea of developing a functional biofertilizer with a long shelf-life
culture media and oxygen transfer were studied to improve the production
of R. phaseoli in chemically defined and undefined media for scale-up
from shake flasks to a pneumatic bubble column bioreactor (BCB). The
effects of carbon and phosphate on R. phaseoli growth were evaluated,
and the specific volumetric oxygen transfer coefficient (k(L)a) was
determined as a criterion to scale-up to BCB. Six-fold more viable
biomass was obtained by increasing the phosphate concentration by
7.5-fold when using glucose. However, this increase was not observed
when succinate was used. In the undefined medium, glucose induced a
higher production of R. phaseoli in comparison with other carbon
sources. The k(L)a of 6-11 h(-1) produced a five-fold growth of biomass
in shake-flask cultures and was used to produce a biofertilizer in BCB.
The formation of nitrogen-fixing nodules was confirmed in bean plants
(Phaseolus vulgaris). The improvement of culture media with a suitable
oxygen transfer demonstrates that R. phaseoli can be used as an
inoculant that exerts beneficial effects on the initial growth of bean
plants.
Filiaciones:
Valdez-Cruz, N. A.:
I , Univ Nacl Autonoma Mexico, Programa Invest Prod Biomol, Unidad Bioproc, Dept Biol Mol & Biotecnol,Inst Invest Biomed, Ciudad De Mexico, Mexico
Daniel-Vazquez, A.:
I , Univ Nacl Autonoma Mexico, Programa Invest Prod Biomol, Unidad Bioproc, Dept Biol Mol & Biotecnol,Inst Invest Biomed, Ciudad De Mexico, Mexico
Blancas-Cabrera, A.:
I , Univ Nacl Autonoma Mexico, Programa Invest Prod Biomol, Unidad Bioproc, Dept Biol Mol & Biotecnol,Inst Invest Biomed, Ciudad De Mexico, Mexico
Trujillo-Rollan, M. A.:
I , Univ Nacl Autonoma Mexico, Programa Invest Prod Biomol, Unidad Bioproc, Dept Biol Mol & Biotecnol,Inst Invest Biomed, Ciudad De Mexico, Mexico
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