The Entry Blocker Peptide Produced in Chlamydomonas reinhardtii Inhibits Influenza Viral Replication in vitro
Por:
Reyes-Barrera, Karen Lizbeth, Soria-Guerra, Ruth Elena, Lopez-Martinez, Rogelio, HUERTA, LEONOR, Salinas-Jazmin, Nohemi, Cabello-Gutierrez, Carlos, Alpuche-Solis, Angel Gabriel
Publicada:
12 may 2021
Categoría:
Plant science
Resumen:
This year, a respiratory virus caused an emergency pandemic alert in
health services around the world, showing the need for biotechnological
approaches to fight these diseases. The influenza virus is one of the
main viral agents that generate pandemic outbreaks. Currently, the
majority of co-circulating influenza A virus (IAV) strains are
adamantine- and oseltamivir-resistant strains, and the challenge is to
find new antivirals for more efficient treatments. The antiviral entry
blocker (EB) peptide is a promising candidate for blocking the virus
entry into cells. The aim of this research was to express the EB peptide
in the microalgae Chlamydomonas reinhardtii and test its antiviral
activity against IAV in vitro. The EB peptide nucleotide sequence was
introduced into the nuclear genome of microalgae using Agrobacterium
tumefaciens transformation. The EB peptide amount produced in
transformed microalgae was 4.99 +/- 0.067% of the total soluble
protein. In hemagglutination inhibition assays using influenza A/H1N1
pdm and influenza A H1N1/Virginia/ATCC/2009 strains, we reported that
the EB peptide extract from the microalgae showed 100-fold higher
efficiency than the EB synthetic peptide. In addition, both the EB
peptide extract and synthetic peptide inhibited viral replication in
MDCK cells (IC50 = 20.7 nM and IC50 = 754.4 nM, respectively); however,
the EB peptide extract showed a 32-fold higher antiviral effectiveness
than the synthetic peptide against influenza A/H1N1 pdm. Extracts from
untransformed and transformed microalgae and synthetic peptide did not
show cytotoxic effect on MDCK cell monolayers. Thus, C. reinhardtii may
be a fast, safe, and effective expression platform for production of
peptides with significant antiviral activity and can be used as a
prophylactic treatment to reduce viral propagation.
Filiaciones:
Reyes-Barrera, Karen Lizbeth:
Inst Potosino Invest Cient & Tecnol AC, Div Mol Biol, Lab Biol Mol Plantas, San Luis Potosi, San Luis Potosi, Mexico
Soria-Guerra, Ruth Elena:
Univ Autonoma San Luis Potosi, Fac Ciencias Quim, Lab Biotecnol Mol Celulas Vegetales, San Luis Potosi, San Luis Potosi, Mexico
Lopez-Martinez, Rogelio:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City, DF, Mexico
HUERTA, LEONOR:
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Mexico City, DF, Mexico
Salinas-Jazmin, Nohemi:
Univ Nacl Autonoma Mexico, Fac Med, Dept Farmacol, Mexico City, DF, Mexico
Cabello-Gutierrez, Carlos:
Inst Nacl Enfermedades Resp Ismael Cosio Villegas, Dept Invest Virol & Micol, Mexico City, DF, Mexico
Alpuche-Solis, Angel Gabriel:
Inst Potosino Invest Cient & Tecnol AC, Div Mol Biol, Lab Biol Mol Plantas, San Luis Potosi, San Luis Potosi, Mexico
Gold, Green Accepted
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