Bioprospecting of wild type ethanologenic yeast for ethanol fuel production from wastewater-grown microalgae
Por:
Romero-Frasca, Enrique, Velasquez-Orta, Sharon B., Escobar-Sanchez, Viviana, Tinoco-Valencia, Raunel, Orta Ledesma, Maria Teresa
Publicada:
9 abr 2021
Resumen:
BackgroundWild-type yeasts have been successfully used to obtain food
products, yet their full potential as fermenting microorganisms for
large-scale ethanol fuel production has to be determined. In this study,
wild-type ethanologenic yeasts isolated from a secondary effluent were
assessed for their capability to ferment saccharified microalgae
sugars.ResultsYeast species in wastewater were identified sequencing the
Internal Transcribed Spacers 1 and 2 regions of the ribosomal cluster.
Concurrently, microalgae biomass sugars were saccharified via acid
hydrolysis, producing 5.00.3 g L-1 of fermentable sugars. Glucose
consumption and ethanol production of yeasts in hydrolyzed-microalgae
liquor were tested at different initial sugar concentrations and
fermentation time. The predominant ethanologenic yeast species was
identified as Candida sp., and glucose consumption for this strain and
S. cerevisiae achieved 75% and 87% of the initial concentration at
optimal conditions, respectively. Relatively similar ethanol yields were
determined for both species, achieving 0.450.05 (S. cerevisiae) and 0.46
+/- 0.05 g ethanol per g glucose (Candida sp.).Conclusion Overall, the
results provide a first insight of the fermentation capacities of
specific wild-type Candida species, and their potential role in ethanol
industries seeking to improve their cost-efficiency.
Filiaciones:
Romero-Frasca, Enrique:
Instituto de Ingeniería, Coordinación de Ingeniería Ambiental, Universidad Nacional Autónoma de México, Apartado Postal 70-472, Coyoacán, Ciudad de México, 04510, Mexico
Velasquez-Orta, Sharon B.:
School of Engineering, Merz Court, Newcastle University, Newcastle upon Tyne, United Kingdom
Escobar-Sanchez, Viviana:
Laboratorio de Biología Molecular Y Genómica, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Tinoco-Valencia, Raunel:
Instituto de Biotecnología, Unidad de Escalamiento Y Planta Piloto, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Orta Ledesma, Maria Teresa:
Instituto de Ingeniería, Coordinación de Ingeniería Ambiental, Universidad Nacional Autónoma de México, Apartado Postal 70-472, Coyoacán, Ciudad de México, 04510, Mexico
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