Micro-mechanical properties of corneal scaffolds from two different bio-models obtained by an efficient chemical decellularization
Por:
Marin-Tapia, Hugo Arian, Romero-Salazar, Lorena, Arteaga-Arcos, Juan Carlos, Rosales-Ibanez, Raul, Mayorga-Rojas, Miguel
Publicada:
1 jul 2021
Resumen:
The present study elucidates the impact of detergent-based chemical
decellularization on the micro-mechanical properties of porcine and
rabbit corneas for the purpose of extracellular matrix (ECM) derived
scaffolds. Aiming to optimize the decellularization process, different
concentrations of Sodium Dodecyl Sulfate (SDS), Triton X-100 and CHAPS
detergents were assessed on their ability to decellularize corneas from
both bio-models at incubation periods of 12 and 24h. We evaluated the
effect of decellularization on corneal ECM Young's Modulus and various
area's roughness parameters (topography features) at a microscale by
using Atomic Force Microscopy (AFM). Only SDS presented adequate
decellularization properties at the selected concentrations (0.2, 0.5
and 1%) and incubation periods. All topography features displayed by
native corneas were preserved after SDS treatments, while no
statistically significant differences were identified for the average
value of Young's Modulus between the control samples and those treated
with 0.2% SDS (rabbit corneas) and 0.5% SDS (porcine corneas) after
12h. In this sense, cornea decellularization procedures can be improved
by simultaneously reducing SDS concentration and incubation period. AFM
is a useful tool to perform biomechanical analysis of the effect of
decellularization on scaffold micro-mechanics. Evaluation of the
scaffold mechanical behavior at a microscale could help in understanding
cell-scaffold interactions in terms of mechanotransduction,
complementing macroscale techniques (e.g. tensile tests) relevant for
tissue engineering quality control and decision-making.
Filiaciones:
Marin-Tapia, Hugo Arian:
Rheology, Nanofluids and Microfluidics Laboratory, Autonomous University of the State of Mexico, Faculty of Sciences, Campus Universitario ‘‘El Cerrillo, Piedras Blancas”, Carretera Toluca-Ixtlahuaca km 15.5, Estado de México, C.P. 50200, Mexico
Autonomous University of the State of Mexico, Faculty of Sciences, Campus Universitario ‘‘El Cerrillo, Piedras Blancas”, Carretera Toluca-Ixtlahuaca km 15.5, Estado de México, C.P. 50200, Mexico
Autonomous Univ State Mexico, Fac Sci, Rheol Nanofluids & Microfluid Lab, Campus Univ El Cerrillo,Carretera Toluca Ixtlahua, Toluca 50200, Estado De Mexic, Mexico
Autonomous Univ State Mexico, Fac Sci, Campus Univ Cerrillo,Carretera,Toluca Ixtlahuaca, Toluca 50200, Estado De Mexic, Mexico
Romero-Salazar, Lorena:
Autonomous University of the State of Mexico, Faculty of Sciences, Campus Universitario ‘‘El Cerrillo, Piedras Blancas”, Carretera Toluca-Ixtlahuaca km 15.5, Estado de México, C.P. 50200, Mexico
Nanothermodynamics and Complex Systems Laboratory, Autonomous University of the State of Mexico, Faculty of Sciences, Campus Universitario ‘‘El Cerrillo, Piedras Blancas”, Carretera Toluca-Ixtlahuaca km 15.5, Estado de México, C.P. 50200, Mexico
Autonomous Univ State Mexico, Nanothermodynam & Complex Syst Lab, Fac Sci, Campus Univ Cerrillo,Carretera,Toluca Ixtlahuaca, Toluca 50200, Estado De Mexic, Mexico
Arteaga-Arcos, Juan Carlos:
Micromechanics Laboratory, Autonomous University of the State of Mexico, Faculty of Sciences, Instituto Literario, No. 100 Ote., C.P, Toluca, State of Mexico 50000, Mexico
Autonomous Univ State Mexico, Micromech Lab, Fac Sci, Inst Literario, 100 Ote, Toluca 50200, State Of Mexico, Mexico
Rosales-Ibanez, Raul:
Tissue Engineering and Translational Medicine Laboratory, Faculty of Higher Studies (FES) Iztacala, National Autonomous University of Mexico, Tlalnepantla, Mexico City, 54090, Mexico
Univ Nacl Autonoma Mexico, Fac Higher Studies FES Iztacala, Tissue Engn & Translat Med Lab, Mexico City 54090, DF, Mexico
Mayorga-Rojas, Miguel:
Rheology, Nanofluids and Microfluidics Laboratory, Autonomous University of the State of Mexico, Faculty of Sciences, Campus Universitario ‘‘El Cerrillo, Piedras Blancas”, Carretera Toluca-Ixtlahuaca km 15.5, Estado de México, C.P. 50200, Mexico
Autonomous University of the State of Mexico, Faculty of Sciences, Campus Universitario ‘‘El Cerrillo, Piedras Blancas”, Carretera Toluca-Ixtlahuaca km 15.5, Estado de México, C.P. 50200, Mexico
Autonomous Univ State Mexico, Fac Sci, Rheol Nanofluids & Microfluid Lab, Campus Univ El Cerrillo,Carretera Toluca Ixtlahua, Toluca 50200, Estado De Mexic, Mexico
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