High-throughput transcriptome sequencing and comparative analysis of Escherichia coli and Schizosaccharomyces pombe in respiratory and fermentative growth
Por:
Vichi, Joivier, Salazar, Emmanuel, Jacinto V.J., Rodriguez L.O., Grande, Ricardo, Dantan-Gonzalez, Edgar, Morett, Enrique, Hernandez-Mendoza, Armando
Publicada:
1 ene 2021
Categoría:
Multidisciplinary
Resumen:
In spite of increased complexity in eukaryotes compared to prokaryotes, several basic metabolic and regulatory processes are conserved. Here we explored analogies in the eubacteria Escherichia coli and the unicellular fission yeast Schizosaccharomyces pombe transcriptomes under two carbon sources: 2% glucose; or a mix of 2% glycerol and 0.2% sodium acetate using the same growth media and growth phase. Overall, twelve RNA-seq libraries were constructed. A total of 593 and 860 genes were detected as differentially expressed for E. coli and S. pombe, respectively, with a log2 of the Fold Change = 1 and False Discovery Rate = 0.05. In aerobic glycolysis, most of the expressed genes were associated with cell proliferation in both organisms, including amino acid metabolism and glycolysis. In contrast in glycerol/acetate condition, genes related to flagellar assembly and membrane proteins were differentially expressed such as the general transcription factors fliA, flhD, flhC, and flagellum assembly genes were detected in E. coli, whereas in S. pombe genes for hexose transporters, integral membrane proteins, galactose metabolism, and ncRNAs related to cellular stress were overexpressed. In general, our study shows that a conserved "foraging behavior" response is observed in these eukaryotic and eubacterial organisms in gluconeogenic carbon sources. Copyright: © 2021 Vichi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Filiaciones:
Vichi, Joivier:
Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico
Univ Autonoma Estado Morelos, Ctr Invest Dinam Celular, Inst Invest Ciencias Basicas & Aplicadas, Cuernavaca, Morelos, Mexico
Salazar, Emmanuel:
Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Univ Autonoma Estado Morelos, Ctr Invest Dinam Celular, Inst Invest Ciencias Basicas & Aplicadas, Cuernavaca, Morelos, Mexico
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca, Morelos, Mexico
Jacinto V.J.:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Rodriguez L.O.:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Grande, Ricardo:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca, Morelos, Mexico
Dantan-Gonzalez, Edgar:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico
Univ Autonoma Estado Morelos, Ctr Invest Biotecnol, Cuernavaca, Morelos, Mexico
Morett, Enrique:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca, Morelos, Mexico
Hernandez-Mendoza, Armando:
Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico
Univ Autonoma Estado Morelos, Ctr Invest Dinam Celular, Inst Invest Ciencias Basicas & Aplicadas, Cuernavaca, Morelos, Mexico
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