Innovative Control of Biofilms on Stainless Steel Surfaces Using Electrolyzed Water in the Dairy Industry


Por: Jimenez-Pichardo, Rodrigo, Hernandez-Martinez, Iriana, Regalado-Gonzalez, Carlos, Santos-Cruz, Jose, Meas-Vong, Yunny, Wacher-Rodarte, Maria del Carmen, Carrillo-Reyes, Julian, Sanchez-Ortega, Irais, Garcia-Almendarez, Blanca Estela

Publicada: 1 ene 2021
Resumen:
Biofilms on food-contact surfaces can lead to recurrent contamination. This work aimed to study the biofilm formation process on stainless steel plates used in the dairy industry: 304 surface finish 2B and electropolished; and the effect of a cleaning and disinfection process using alkaline (AEW) and neutral (NEW) electrolyzed water. Milk fouling during heat processing can lead to type A or B deposits, which were analyzed for composition, surface energy, thickness, and roughness, while the role of raw milk microbiota on biofilm development was investigated. Bacteria, yeasts, and lactic acid bacteria were detected using EUB-338, PF2, and Str-493 probes, respectively, whereas Lis-637 probe detected Listeria sp. The genetic complexity and diversity of biofilms varied according to biofilm maturation day, as evaluated by 16S rRNA gene sequence, denaturing gradient gel electrophoresis, and fluorescence in situ hybridization microscopy. From analysis of the experimental designs, a cleaning stage of 50 mg/L NaOH of AEW at 30 degrees C for 10 min, followed by disinfection using 50 mg/L total available chlorine of NEW at 20 degrees C for 5 min is a sustainable alternative process to prevent biofilm formation. Fluorescence microscopy was used to visualize the effectiveness of this process.

Filiaciones:
Jimenez-Pichardo, Rodrigo:
 Technol Univ Mineral Reforma, Camino Providencia La Calera 1000, Mineral De Reforma 42186, Hidalgo, Mexico

Hernandez-Martinez, Iriana:
 Autonomous Univ Queretaro CU, Fac Chem, PROPAC, DIPA, Cerro Campanas Col Las Campanas S-N, Queretaro Qro 76010, Mexico

Regalado-Gonzalez, Carlos:
 Autonomous Univ Queretaro CU, Fac Chem, PROPAC, DIPA, Cerro Campanas Col Las Campanas S-N, Queretaro Qro 76010, Mexico

Santos-Cruz, Jose:
 Autonomous Univ Queretaro CU, Fac Chem, PROPAC, DIPA, Cerro Campanas Col Las Campanas S-N, Queretaro Qro 76010, Mexico

Meas-Vong, Yunny:
 CIDETEQ, Ctr Res & Dev Electrochem, Parque Tecnol Queretaro Sanfandila, Pedro Escobedo 76703, Queretaro, Mexico

Wacher-Rodarte, Maria del Carmen:
 Univ Nacl Autonoma Mexico, Fac Chem, Food & Biotechnol Dept, Av Univ 3000,Circuito Exterior S-N, Mexico City 04510, DF, Mexico

Carrillo-Reyes, Julian:
 Univ Nacl Autonoma Mexico, Engn Inst, Acad Unit Juriquilla, Blvd Juriquilla 3001, Juriquilla 76230, Queretaro, Mexico

Sanchez-Ortega, Irais:
 Autonomous Univ Hidalgo State, Basic Sci & Engn Inst, Carr Pachuca Tulancingo Km 4-5, Mineral Reforma Hidalgo 42184, Mexico

Garcia-Almendarez, Blanca Estela:
 Autonomous Univ Queretaro CU, Fac Chem, PROPAC, DIPA, Cerro Campanas Col Las Campanas S-N, Queretaro Qro 76010, Mexico
ISSN: 23048158
Editorial
MDPI Multidisciplinary Digital Publishing Institute, ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND, Suiza
Tipo de documento: Article
Volumen: 10 Número: 1
Páginas:
WOS Id: 000610233400001
ID de PubMed: 33419000

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