Innovative Control of Biofilms on Stainless Steel Surfaces Using Electrolyzed Water in the Dairy Industry
Por:
Jimenez-Pichardo, Rodrigo, Hernandez-Martinez, Iriana, Regalado-Gonzalez, Carlos, Santos-Cruz, Jose, Meas-Vong, Yunny, Wacher-Rodarte, Maria del Carmen, Carrillo-Reyes, Julian, Sanchez-Ortega, Irais, Garcia-Almendarez, Blanca Estela
Publicada:
1 ene 2021
Resumen:
Biofilms on food-contact surfaces can lead to recurrent contamination.
This work aimed to study the biofilm formation process on stainless
steel plates used in the dairy industry: 304 surface finish 2B and
electropolished; and the effect of a cleaning and disinfection process
using alkaline (AEW) and neutral (NEW) electrolyzed water. Milk fouling
during heat processing can lead to type A or B deposits, which were
analyzed for composition, surface energy, thickness, and roughness,
while the role of raw milk microbiota on biofilm development was
investigated. Bacteria, yeasts, and lactic acid bacteria were detected
using EUB-338, PF2, and Str-493 probes, respectively, whereas Lis-637
probe detected Listeria sp. The genetic complexity and diversity of
biofilms varied according to biofilm maturation day, as evaluated by 16S
rRNA gene sequence, denaturing gradient gel electrophoresis, and
fluorescence in situ hybridization microscopy. From analysis of the
experimental designs, a cleaning stage of 50 mg/L NaOH of AEW at 30
degrees C for 10 min, followed by disinfection using 50 mg/L total
available chlorine of NEW at 20 degrees C for 5 min is a sustainable
alternative process to prevent biofilm formation. Fluorescence
microscopy was used to visualize the effectiveness of this process.
Filiaciones:
Jimenez-Pichardo, Rodrigo:
Technol Univ Mineral Reforma, Camino Providencia La Calera 1000, Mineral De Reforma 42186, Hidalgo, Mexico
Hernandez-Martinez, Iriana:
Autonomous Univ Queretaro CU, Fac Chem, PROPAC, DIPA, Cerro Campanas Col Las Campanas S-N, Queretaro Qro 76010, Mexico
Regalado-Gonzalez, Carlos:
Autonomous Univ Queretaro CU, Fac Chem, PROPAC, DIPA, Cerro Campanas Col Las Campanas S-N, Queretaro Qro 76010, Mexico
Santos-Cruz, Jose:
Autonomous Univ Queretaro CU, Fac Chem, PROPAC, DIPA, Cerro Campanas Col Las Campanas S-N, Queretaro Qro 76010, Mexico
Meas-Vong, Yunny:
CIDETEQ, Ctr Res & Dev Electrochem, Parque Tecnol Queretaro Sanfandila, Pedro Escobedo 76703, Queretaro, Mexico
Wacher-Rodarte, Maria del Carmen:
Univ Nacl Autonoma Mexico, Fac Chem, Food & Biotechnol Dept, Av Univ 3000,Circuito Exterior S-N, Mexico City 04510, DF, Mexico
Carrillo-Reyes, Julian:
Univ Nacl Autonoma Mexico, Engn Inst, Acad Unit Juriquilla, Blvd Juriquilla 3001, Juriquilla 76230, Queretaro, Mexico
Sanchez-Ortega, Irais:
Autonomous Univ Hidalgo State, Basic Sci & Engn Inst, Carr Pachuca Tulancingo Km 4-5, Mineral Reforma Hidalgo 42184, Mexico
Garcia-Almendarez, Blanca Estela:
Autonomous Univ Queretaro CU, Fac Chem, PROPAC, DIPA, Cerro Campanas Col Las Campanas S-N, Queretaro Qro 76010, Mexico
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